P53-DNA Recognition: Difference between revisions
m Sandbox Reserved 170 moved to P53-DNA Recognition: requested by author |
No edit summary |
||
| Line 1: | Line 1: | ||
''This is a joint project of La Canada High School and University of Southern California students, mentored by Professor Remo Rohs.'' | ''This is a joint project of La Canada High School and University of Southern California students, mentored by Professor Remo Rohs.'' | ||
| Line 61: | Line 57: | ||
The distinct <scene name='Sandbox_Reserved_170/Arg248/2'>shape of the minor groove recognized by Arg248</scene> is due to a transition of the four A/T base pairs of the CATG core elements to a Hoogsteen base pairing geometry. Regions with Hoogsteen base pairs (green) <scene name='Sandbox_Reserved_170/Hg_helix/2'>decrease the diameter of the double helix</scene> compared to regions with Watson-Crick base pairs (blue). | The distinct <scene name='Sandbox_Reserved_170/Arg248/2'>shape of the minor groove recognized by Arg248</scene> is due to a transition of the four A/T base pairs of the CATG core elements to a Hoogsteen base pairing geometry. Regions with Hoogsteen base pairs (green) <scene name='Sandbox_Reserved_170/Hg_helix/2'>decrease the diameter of the double helix</scene> compared to regions with Watson-Crick base pairs (blue). | ||
The reason for this deformation of the double helix is the <scene name='Group:USC-LCHS/3kz8_ba_hoogsteencloseup/2'>base pairing geometry in Hoogsteen base pairs</scene> with the approximately 180 degree rotation of adenine around the glycosidic bond and formation of hydrogen bonds with thymine at a different edge of the adenine base compared to <scene name='Group:USC-LCHS/3kmd_wcbp_closeup/1'>standard Watson-Crick base pairing</scene>, depicted here for the identical base pair in a p53 response element with different sequence from [ | The reason for this deformation of the double helix is the <scene name='Group:USC-LCHS/3kz8_ba_hoogsteencloseup/2'>base pairing geometry in Hoogsteen base pairs</scene> with the approximately 180 degree rotation of adenine around the glycosidic bond and formation of hydrogen bonds with thymine at a different edge of the adenine base compared to <scene name='Group:USC-LCHS/3kmd_wcbp_closeup/1'>standard Watson-Crick base pairing</scene>, depicted here for the identical base pair in a p53 response element with different sequence from [[3kmd|PDB ID# 3KMD]]. | ||
=Further Reading= | =Further Reading= | ||