P53-DNA Recognition: Difference between revisions

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

Jaime Prilusky, Remo Rohs, Bailey Holmes, Ana Carolina Dantas Machado, Eran Hodis, Julia Tam, Masha Karelina, Sharon Kim, Skyler Saleebyan, Keziah Kim, Joseph M. Steinberger, Eric Martz, Alexander Berchansky, Michal Harel, Angel Herraez, Joel L. Sussman

@@ Line 9: / Line 9: @@
 [[Image:p53-intro.jpg|thumb|left|300px|Figure 1: Crystal structure of a p53 DBD tetramer-DNA complex; PDB ID# 3KZ8 <ref name='kitayner'>Kitayner M, Rozenberg H, Rohs R, Suad O, Rabinovich D, Honig B, Shakked Z. Diversity in DNA recognition by p53 revealed by crystal structures with Hoogsteen base pairs. Nat Struct Mol Biol. 2010;17(4):423-9.</ref>.]]
-[[Image:p53-consensus.jpg|thumb|right|400px|consensus site]]
+[[Image:p53-consensus.jpg|thumb|right|400px|Figure 2: p53 consensus site with R= A or G, Y= C or T, and W=A or T.]]
 [[Image:p53-domains.jpg|thumb|right|300px|domains]]
-Also known as the '''Guardian of the Genome''', the tumor suppressor p53 is central in the natural defense against human cancer. The protein is activated by stress factors that can compromise the genomic integrity of the cell, and this activation unleashes the function of p53 as transcription factor. It binds as a tetramer (Figure 1) to a large range of DNA response elements, which are formed by two decameric half-sites that are separated by a variable number of base pairs (Figure2). Binding of p53 to different response elements leads to distinct biological responses, such as cell-cycle arrest, senescence or apoptosis.
+Also known as the '''Guardian of the Genome''', the tumor suppressor p53 is central in the natural defense against human cancer. The protein is activated by stress factors that can compromise the genomic integrity of the cell, and this activation unleashes the function of p53 as transcription factor. It binds as a tetramer (Figure 1) to a large range of DNA response elements. The p53 consensus site  (Figure 2) is formed by two decameric half-sites, each containing a core element (red), that are separated by a variable number of base pairs (blue). Binding of p53 to different response elements leads to distinct biological responses, such as cell-cycle arrest, senescence or apoptosis.