Vibrio cholerae colonization factor TcpF: Difference between revisions

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Yang Yang, Alexander Berchansky, Michal Harel

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 [[Image:RNA.png|left|300px|thumb| TcpF-RNA [[1xtc]]]]
-The structure of TcpF is consisted with an <scene name='Vibrio_cholerae_colonization_factor_TcpF/N_terminal_domain/1'>N terminal domain (NTD;residues 1 to 185)</scene> and a <scene name='Vibrio_cholerae_colonization_factor_TcpF/Ctd/1'>C terminal domain (CTD; residues 190 to 318)</scene> connected by an extended <scene name='Vibrio_cholerae_colonization_factor_TcpF/Linker_1/1'> linker segment (residues 186 to 189)</scene>. In detail, the NTD is composed of a short twisted β-sheet encapsulated by seven short α-helices with a second twisted β-sheet forming the floor of this domain. The NTD is connected with The CTD, which consists of two twisted antiparallel β-sheets.<ref name="map" /> TCP is a filamentous structure belongs. Studies showed that the regions, close to CTD  are important for mediating colonization. The architecture of TcpF, with discrete NTD and CTD joined by a linker, is flexible that allow to accommodate a larger substrate. By looking at the homology of TcpF, the NTD and the CTD together form a unique interface that interacts with partner proteins to function in V. cholerae colonization.<ref>TcpF Is a Soluble Colonization Factor and Protective Antigen Secreted by El Tor and Classical O1 and O139 Vibrio cholerae Serogroups  Thomas J. Kirn and Ronald K. Taylor [http://iai.asm.org/content/73/8/4461 DOI: 10.1128/IAI.73.8.4461-4470.2005] Infect. Immun. August 2005 vol. 73 no. 8 4461-4470.</ref>
+The structure of TcpF is consisted with an <scene name='Vibrio_cholerae_colonization_factor_TcpF/N_terminal_domain/1'>N terminal domain (NTD;residues 1 to 185)</scene> and a <scene name='Vibrio_cholerae_colonization_factor_TcpF/Ctd/1'>C terminal domain (CTD; residues 190 to 318)</scene> connected by an extended <scene name='Vibrio_cholerae_colonization_factor_TcpF/Linker_1/1'> linker segment (residues 186 to 189)</scene>. In detail, the NTD is composed of a short twisted β-sheet encapsulated by seven short α-helices with a second twisted β-sheet forming the floor of this domain. The NTD is connected with The CTD, which consists of two twisted antiparallel β-sheets.<ref name="map" /> TCP is a filamentous structure belongs. Studies showed that the regions, close to CTD  are important for mediating colonization. The architecture of TcpF, with discrete NTD and CTD joined by a linker, is flexible that allow to accommodate a larger substrate.
 == Function ==
 {{STRUCTURE_3oc5|  PDB=3oc5  | SIZE=300| SCENE=Vibrio_cholerae_colonization_factor_TcpF/Consurf/1 |right|CAPTION=Consurf image [[3oc5]] }}
-The function of many of Tcp genes and their associated proteins are largely unknown. TcpF is the only protein secreted by the TCP apparatus and it represents the first nonpilus protein identified that is specifically secreted outside the bacterial cell by a type IV pilus biogenesis apparatus.<ref name="map" /> Studies hypothesize that TcpF, identified in classical isolates of V. cholerae O1 is an essential factor for colonization in the infant mouse cholera model. Bacteria lacking tcpF are deficient in colonization, and anti-TcpF antibodies are protective in the infant mouse cholera model. TcpF is expressed in vivo during human infection and generates a substantial immune response in patients infected with V. cholera.
+The function of many of Tcp genes and their associated proteins are largely unknown. TcpF is the only protein secreted by the TCP apparatus and it represents the first nonpilus protein identified that is specifically secreted outside the bacterial cell by a type IV pilus biogenesis apparatus.<ref name="map" /> Studies hypothesize that TcpF, identified in classical isolates of V. cholerae O1 is an essential factor for colonization in the infant mouse cholera model. Bacteria lacking tcpF are deficient in colonization, and anti-TcpF antibodies are protective in the infant mouse cholera model. TcpF is expressed in vivo during human infection and generates a substantial immune response in patients infected with V. cholera. By looking at the homology of TcpF, the NTD and the CTD together form a unique interface that interacts with partner proteins to function in V. cholerae colonization.<ref>TcpF Is a Soluble Colonization Factor and Protective Antigen Secreted by El Tor and Classical O1 and O139 Vibrio cholerae Serogroups  Thomas J. Kirn and Ronald K. Taylor [http://iai.asm.org/content/73/8/4461 DOI: 10.1128/IAI.73.8.4461-4470.2005] Infect. Immun. August 2005 vol. 73 no. 8 4461-4470.</ref>
 == Evolution ==