Sandbox Reserved 497: Difference between revisions

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OCA, Kara Tinker

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 A proposed mechanism for the methyl transfer reaction catalyzed by DmdA has recently been published based on the known structural characteristics of DmdA as well as what is known about similar reactions, namely redox-neutral methyl transfer reactions involving THF as well reactions that involve a methyl transfer from a sulfonium atom to a nitrogen atom
-<ref> Schuller, D.J., Reisch, C.R., Moran, M.A., Whitman, W.B., Lanzilotta, W.N. (2012) Structures of dimethylsulfoniopropinate-dependent demethylase from the marine organism pelagabacter ubique. Protein Sci. 21: 289-298. </ref>.
+<ref> Schuller, D.J., Reisch, C.R., Moran, M.A., Whitman, W.B., Lanzilotta, W.N. (2012) Structures of dimethylsulfoniopropinate-dependent demethylase from the marine organism pelagabacter ubique. Protein Sci. 21: 289-298. </ref>. The proposed mechanism involves an SN2 intermediate with a concerted methyl and a proton transfer mediated by a water molecule present in the active site. This proposed reaction seems likely due to the location of the active site, which is highly acessible by water, as well as the <scene name='Sandbox_Reserved_497/Acidicactivesite/1'>acidic side chains </scene> present in the active site. Additionally DmdA contains a sulfonium atom which tends to make methyl a fairly good leaving group.
-The proposed mechanism involves an SN2 intermediate with a concerted methyl and a proton transfer mediated by a water molecule present in the active site. This proposed reaction seems likely due to the location of the active site, which is highly acessible by water, as well as the <scene name='Sandbox_Reserved_497/Acidicactivesite/1'>acidic side chains </scene> present in the active site. Additionally DmdA contains a sulfonium atom which tends to make methyl a fairly good leaving group.
+[[Image:DmdA_Mechanism.jpg|thumb|550px|right|The proposed mechanism for the methyl transfer reaction catalyzed by DmdA. This image was obtained directly from Schuller et al.]]
-[[Image:DmdA_Mechanism.jpg|thumb|600px|right|The proposed mechanism for the methyl transfer reaction catalyzed by DmdA. This image was obtained directly from Schuller et al.]]
 ==Possible Applications==