1kqo: Difference between revisions

Revision as of 14:36, 21 February 2008

Crystal structure of NMN/NaMN adenylyltransferase complexed with deamido-NAD

OverviewOverview

Nicotinamide/nicotinate mononucleotide (NMN/ NaMN)adenylyltransferase (NMNAT) is an indispensable enzyme in the biosynthesis of NAD(+) and NADP(+). Human NMNAT displays unique dual substrate specificity toward both NMN and NaMN, thus flexible in participating in both de novo and salvage pathways of NAD synthesis. Human NMNAT also catalyzes the rate-limiting step of the metabolic conversion of the anticancer agent tiazofurin to its active form tiazofurin adenine dinucleotide (TAD). The tiazofurin resistance is mainly associated with the low NMNAT activity in the cell. We have solved the crystal structures of human NMNAT in complex with NAD, deamido-NAD, and a non-hydrolyzable TAD analogue beta-CH(2)-TAD. These complex structures delineate the broad substrate specificity of the enzyme toward both NMN and NaMN and reveal the structural mechanism for adenylation of tiazofurin nucleotide. The crystal structure of human NMNAT also shows that it forms a barrel-like hexamer with the predicted nuclear localization signal sequence located on the outside surface of the barrel, supporting its functional role of interacting with the nuclear transporting proteins. The results from the analytical ultracentrifugation studies are consistent with the formation of a hexamer in solution under certain conditions.

About this StructureAbout this Structure

1KQO is a Single protein structure of sequence from Homo sapiens with as ligand. Active as Nicotinamide-nucleotide adenylyltransferase, with EC number 2.7.7.1 Full crystallographic information is available from OCA.

ReferenceReference

Structure of human nicotinamide/nicotinic acid mononucleotide adenylyltransferase. Basis for the dual substrate specificity and activation of the oncolytic agent tiazofurin., Zhou T, Kurnasov O, Tomchick DR, Binns DD, Grishin NV, Marquez VE, Osterman AL, Zhang H, J Biol Chem. 2002 Apr 12;277(15):13148-54. Epub 2002 Jan 11. PMID:11788603

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OCA

@@ Line 4: / Line 4: @@
 ==Overview==
-Nicotinamide/nicotinate mononucleotide (NMN/ NaMN)adenylyltransferase, (NMNAT) is an indispensable enzyme in the biosynthesis of NAD(+) and, NADP(+). Human NMNAT displays unique dual substrate specificity toward, both NMN and NaMN, thus flexible in participating in both de novo and, salvage pathways of NAD synthesis. Human NMNAT also catalyzes the, rate-limiting step of the metabolic conversion of the anticancer agent, tiazofurin to its active form tiazofurin adenine dinucleotide (TAD). The, tiazofurin resistance is mainly associated with the low NMNAT activity in, the cell. We have solved the crystal structures of human NMNAT in complex, with NAD, deamido-NAD, and a non-hydrolyzable TAD analogue beta-CH(2)-TAD., These complex structures delineate the broad substrate specificity of the, enzyme toward both NMN and NaMN and reveal the structural mechanism for, adenylation of tiazofurin nucleotide. The crystal structure of human NMNAT, also shows that it forms a barrel-like hexamer with the predicted nuclear, localization signal sequence located on the outside surface of the barrel, supporting its functional role of interacting with the nuclear, transporting proteins. The results from the analytical ultracentrifugation, studies are consistent with the formation of a hexamer in solution under, certain conditions.
+Nicotinamide/nicotinate mononucleotide (NMN/ NaMN)adenylyltransferase (NMNAT) is an indispensable enzyme in the biosynthesis of NAD(+) and NADP(+). Human NMNAT displays unique dual substrate specificity toward both NMN and NaMN, thus flexible in participating in both de novo and salvage pathways of NAD synthesis. Human NMNAT also catalyzes the rate-limiting step of the metabolic conversion of the anticancer agent tiazofurin to its active form tiazofurin adenine dinucleotide (TAD). The tiazofurin resistance is mainly associated with the low NMNAT activity in the cell. We have solved the crystal structures of human NMNAT in complex with NAD, deamido-NAD, and a non-hydrolyzable TAD analogue beta-CH(2)-TAD. These complex structures delineate the broad substrate specificity of the enzyme toward both NMN and NaMN and reveal the structural mechanism for adenylation of tiazofurin nucleotide. The crystal structure of human NMNAT also shows that it forms a barrel-like hexamer with the predicted nuclear localization signal sequence located on the outside surface of the barrel, supporting its functional role of interacting with the nuclear transporting proteins. The results from the analytical ultracentrifugation studies are consistent with the formation of a hexamer in solution under certain conditions.
 ==About this Structure==
@@ Line 14: / Line 14: @@
 [[Category: Nicotinamide-nucleotide adenylyltransferase]]
 [[Category: Single protein]]
-[[Category: Binns, D.D.]]
+[[Category: Binns, D D.]]
-[[Category: Grishin, N.V.]]
+[[Category: Grishin, N V.]]
 [[Category: Kurnasov, O.]]
-[[Category: Marquez, V.E.]]
+[[Category: Marquez, V E.]]
-[[Category: Osterman, A.L.]]
+[[Category: Osterman, A L.]]
-[[Category: Tomchick, D.R.]]
+[[Category: Tomchick, D R.]]
 [[Category: Zhang, H.]]
 [[Category: Zhou, T.]]
@@ Line 25: / Line 25: @@
 [[Category: nucleotidyltransferase superfamily]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri Feb 15 16:15:00 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:36:56 2008''