1jhj: Difference between revisions
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==Overview== | ==Overview== | ||
The anaphase-promoting complex (APC), or cyclosome, is a cell | The anaphase-promoting complex (APC), or cyclosome, is a cell cycle-regulated ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC is composed of at least 11 subunits; no structure has been determined for any of these subunits. The subunit APC10/DOC1, a one-domain protein consisting of 185 amino acids, has a conserved core (residues 22-161) that is homologous to domains found in several other putative ubiquitin ligases and, therefore, may play a role in ubiquitination reactions. Here we report the crystal structure of human APC10 at 1.6 A resolution. The core of the protein is formed by a beta-sandwich that adopts a jellyroll fold. Unexpectedly, this structure is highly similar to ligand-binding domains of several bacterial and eukaryotic proteins, such as galactose oxidase and coagulation factor Va, raising the possibility that APC10 may function by binding a yet unidentified ligand. We further provide biochemical evidence that the C-terminus of APC10 binds to CDC27/APC3, an APC subunit that contains multiple tetratrico peptide repeats. | ||
==About this Structure== | ==About this Structure== | ||
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[[Category: Huber, R.]] | [[Category: Huber, R.]] | ||
[[Category: Jacob, U.]] | [[Category: Jacob, U.]] | ||
[[Category: Peters, J | [[Category: Peters, J M.]] | ||
[[Category: Sondermann, P.]] | [[Category: Sondermann, P.]] | ||
[[Category: Vodermaier, H | [[Category: Vodermaier, H C.]] | ||
[[Category: Wendt, K | [[Category: Wendt, K S.]] | ||
[[Category: NI]] | [[Category: NI]] | ||
[[Category: beta sandwich]] | [[Category: beta sandwich]] | ||
[[Category: jellyroll]] | [[Category: jellyroll]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:22:45 2008'' | ||
Revision as of 14:22, 21 February 2008
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Crystal structure of the APC10/Doc1 subunit of the human anaphase-promoting complex
OverviewOverview
The anaphase-promoting complex (APC), or cyclosome, is a cell cycle-regulated ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC is composed of at least 11 subunits; no structure has been determined for any of these subunits. The subunit APC10/DOC1, a one-domain protein consisting of 185 amino acids, has a conserved core (residues 22-161) that is homologous to domains found in several other putative ubiquitin ligases and, therefore, may play a role in ubiquitination reactions. Here we report the crystal structure of human APC10 at 1.6 A resolution. The core of the protein is formed by a beta-sandwich that adopts a jellyroll fold. Unexpectedly, this structure is highly similar to ligand-binding domains of several bacterial and eukaryotic proteins, such as galactose oxidase and coagulation factor Va, raising the possibility that APC10 may function by binding a yet unidentified ligand. We further provide biochemical evidence that the C-terminus of APC10 binds to CDC27/APC3, an APC subunit that contains multiple tetratrico peptide repeats.
About this StructureAbout this Structure
1JHJ is a Single protein structure of sequence from Homo sapiens with as ligand. Full crystallographic information is available from OCA.
ReferenceReference
Crystal structure of the APC10/DOC1 subunit of the human anaphase-promoting complex., Wendt KS, Vodermaier HC, Jacob U, Gieffers C, Gmachl M, Peters JM, Huber R, Sondermann P, Nat Struct Biol. 2001 Sep;8(9):784-8. PMID:11524682
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