1i2m: Difference between revisions
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==Overview== | ==Overview== | ||
RCC1 (regulator of chromosome condensation), a beta propeller | RCC1 (regulator of chromosome condensation), a beta propeller chromatin-bound protein, is the guanine nucleotide exchange factor (GEF) for the nuclear GTP binding protein Ran. We report here the 1.8 A crystal structure of a Ran*RCC1 complex in the absence of nucleotide, an intermediate in the multistep GEF reaction. In contrast to previous structures, the phosphate binding region of the nucleotide binding site is perturbed only marginally, possibly due to the presence of a polyvalent anion in the P loop. Biochemical experiments show that a sulfate ion stabilizes the Ran*RCC1 complex and inhibits dissociation by guanine nucleotides. Based on the available structural and biochemical evidence, we present a unified scenario for the GEF mechanism where interaction of the P loop lysine with an acidic residue is a crucial element for the overall reaction. | ||
==Disease== | ==Disease== | ||
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[[Category: g fold or gtpase fold]] | [[Category: g fold or gtpase fold]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:07:17 2008'' | ||
Revision as of 14:07, 21 February 2008
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RAN-RCC1-SO4 COMPLEX
OverviewOverview
RCC1 (regulator of chromosome condensation), a beta propeller chromatin-bound protein, is the guanine nucleotide exchange factor (GEF) for the nuclear GTP binding protein Ran. We report here the 1.8 A crystal structure of a Ran*RCC1 complex in the absence of nucleotide, an intermediate in the multistep GEF reaction. In contrast to previous structures, the phosphate binding region of the nucleotide binding site is perturbed only marginally, possibly due to the presence of a polyvalent anion in the P loop. Biochemical experiments show that a sulfate ion stabilizes the Ran*RCC1 complex and inhibits dissociation by guanine nucleotides. Based on the available structural and biochemical evidence, we present a unified scenario for the GEF mechanism where interaction of the P loop lysine with an acidic residue is a crucial element for the overall reaction.
DiseaseDisease
Known diseases associated with this structure: Alveolar soft-part sarcoma OMIM:[606236], Osteolysis, familial expansile OMIM:[603499], Paget disease of bone OMIM:[603499]
About this StructureAbout this Structure
1I2M is a Protein complex structure of sequences from Homo sapiens with as ligand. Full crystallographic information is available from OCA.
ReferenceReference
Structural basis for guanine nucleotide exchange on Ran by the regulator of chromosome condensation (RCC1)., Renault L, Kuhlmann J, Henkel A, Wittinghofer A, Cell. 2001 Apr 20;105(2):245-55. PMID:11336674
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