1fq1: Difference between revisions
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==Overview== | ==Overview== | ||
The CDK-interacting protein phosphatase KAP dephosphorylates | The CDK-interacting protein phosphatase KAP dephosphorylates phosphoThr-160 (pThr-160) of the CDK2 activation segment, the site of regulatory phosphorylation that is essential for kinase activity. Here we describe the crystal structure of KAP in association with pThr-160-CDK2, representing an example of a protein phosphatase in complex with its intact protein substrate. The major protein interface between the two molecules is formed by the C-terminal lobe of CDK2 and the C-terminal helix of KAP, regions remote from the kinase-activation segment and the KAP catalytic site. The kinase-activation segment interacts with the catalytic site of KAP almost entirely via the phosphate group of pThr-160. This interaction requires that the activation segment is unfolded and drawn away from the kinase molecule, inducing a conformation of CDK2 similar to the activated state observed in the CDK2/cyclin A complex. | ||
==About this Structure== | ==About this Structure== | ||
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[[Category: Protein-tyrosine-phosphatase]] | [[Category: Protein-tyrosine-phosphatase]] | ||
[[Category: Barford, D.]] | [[Category: Barford, D.]] | ||
[[Category: Brown, N | [[Category: Brown, N R.]] | ||
[[Category: Hanlon, N.]] | [[Category: Hanlon, N.]] | ||
[[Category: Johnson, L | [[Category: Johnson, L N.]] | ||
[[Category: Noble, M | [[Category: Noble, M E.M.]] | ||
[[Category: Song, H.]] | [[Category: Song, H.]] | ||
[[Category: ATP]] | [[Category: ATP]] | ||
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[[Category: phospho-protein/protein complex]] | [[Category: phospho-protein/protein complex]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:41:21 2008'' | ||