3tg3: Difference between revisions
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[[ | ==Crystal structure of the MAPK binding domain of MKP7== | ||
<StructureSection load='3tg3' size='340' side='right' caption='[[3tg3]], [[Resolution|resolution]] 2.67Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[3tg3]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TG3 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3TG3 FirstGlance]. <br> | |||
</td></tr><tr><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene><br> | |||
<tr><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3tg1|3tg1]]</td></tr> | |||
<tr><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">DUSP16, KIAA1700, MKP7 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])</td></tr> | |||
<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3tg3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tg3 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3tg3 RCSB], [http://www.ebi.ac.uk/pdbsum/3tg3 PDBsum]</span></td></tr> | |||
<table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
The mitogen-activated protein kinase (MAPK) cascades play a pivotal role in a myriad of cellular functions. The specificity and efficiency of MAPK signaling are controlled by docking interactions between MAPKs and their cognate proteins. Many MAPK-interacting partners, including substrates, MAPK kinases, phosphatases, and scaffolding proteins, have linear sequence motifs that mediate the interaction with the common docking site on MAPKs. We report the crystal structure of p38alpha in complex with the MAPK binding domain (KBD) from MAPK phosphatase 5 (MKP5) at 2.7 A resolution. In contrast to the well-known docking mode, the KBD binds p38alpha in a bipartite manner, in which two distinct helical regions of KBD engage the p38alpha docking site, which is situated on the back of the p38alpha active site. We also determined the crystal structure of the KBD of MKP7, which closely resembles the MKP5 KBD, suggesting that the mechanism of molecular recognition by the KBD of MKP5 is conserved in the cytoplasmic p38- and c-Jun N-terminal kinase-specific MKP subgroup. This previously unknown binding mode provides new insights into how MAPKs interact with their binding partners to achieve functional specificity. | |||
A Distinct Interaction Mode Revealed by the Crystal Structure of the Kinase p38alpha with the MAPK Binding Domain of the Phosphatase MKP5.,Zhang YY, Wu JW, Wang ZX Sci Signal. 2011 Dec 20;4(204):ra88. PMID:22375048<ref>PMID:22375048</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
== | |||
< | |||
[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Liu, X.]] | [[Category: Liu, X.]] | ||
Revision as of 08:49, 5 June 2014
Crystal structure of the MAPK binding domain of MKP7Crystal structure of the MAPK binding domain of MKP7
Structural highlights
Publication Abstract from PubMedThe mitogen-activated protein kinase (MAPK) cascades play a pivotal role in a myriad of cellular functions. The specificity and efficiency of MAPK signaling are controlled by docking interactions between MAPKs and their cognate proteins. Many MAPK-interacting partners, including substrates, MAPK kinases, phosphatases, and scaffolding proteins, have linear sequence motifs that mediate the interaction with the common docking site on MAPKs. We report the crystal structure of p38alpha in complex with the MAPK binding domain (KBD) from MAPK phosphatase 5 (MKP5) at 2.7 A resolution. In contrast to the well-known docking mode, the KBD binds p38alpha in a bipartite manner, in which two distinct helical regions of KBD engage the p38alpha docking site, which is situated on the back of the p38alpha active site. We also determined the crystal structure of the KBD of MKP7, which closely resembles the MKP5 KBD, suggesting that the mechanism of molecular recognition by the KBD of MKP5 is conserved in the cytoplasmic p38- and c-Jun N-terminal kinase-specific MKP subgroup. This previously unknown binding mode provides new insights into how MAPKs interact with their binding partners to achieve functional specificity. A Distinct Interaction Mode Revealed by the Crystal Structure of the Kinase p38alpha with the MAPK Binding Domain of the Phosphatase MKP5.,Zhang YY, Wu JW, Wang ZX Sci Signal. 2011 Dec 20;4(204):ra88. PMID:22375048[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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