2pc5: Difference between revisions

Revision as of 19:28, 21 February 2008

Native crystal structure analysis on Arabidopsis dUTPase

OverviewOverview

The deoxyuridine triphosphate nucleotidohydrolase gene from Arabidopsis thaliana was expressed and the gene product was purified. Crystallization was performed by the hanging-drop vapour-diffusion method at 298 K using 2 M ammonium sulfate as the precipitant. X-ray diffraction data were collected to 2.2 A resolution using Cu K alpha radiation. The crystal belongs to the orthorhombic space group P2(1)2(1)2(1), with unit-cell parameters a = 69.90, b = 70.86 A, c = 75.55 A. Assuming the presence of a trimer in the asymmetric unit, the solvent content was 30%, with a V(M) of 1.8 A3 Da(-1).

About this StructureAbout this Structure

2PC5 is a Single protein structure of sequence from Arabidopsis thaliana with as ligand. Active as dUTP diphosphatase, with EC number 3.6.1.23 Known structural/functional Site: . Full crystallographic information is available from OCA.

ReferenceReference

Purification, crystallization and preliminary crystallographic analysis of deoxyuridine triphosphate nucleotidohydrolase from Arabidopsis thaliana., Bajaj M, Moriyama H, Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 May 1;63(Pt, 5):409-11. Epub 2007 Apr 14. PMID:17565183

Page seeded by OCA on Thu Feb 21 18:28:07 2008

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 ==Overview==
-The deoxyuridine triphosphate nucleotidohydrolase gene from Arabidopsis, thaliana was expressed and the gene product was purified. Crystallization, was performed by the hanging-drop vapour-diffusion method at 298 K using 2, M ammonium sulfate as the precipitant. X-ray diffraction data were, collected to 2.2 A resolution using Cu K alpha radiation. The crystal, belongs to the orthorhombic space group P2(1)2(1)2(1), with unit-cell, parameters a = 69.90, b = 70.86 A, c = 75.55 A. Assuming the presence of a, trimer in the asymmetric unit, the solvent content was 30%, with a V(M) of, 1.8 A3 Da(-1).
+The deoxyuridine triphosphate nucleotidohydrolase gene from Arabidopsis thaliana was expressed and the gene product was purified. Crystallization was performed by the hanging-drop vapour-diffusion method at 298 K using 2 M ammonium sulfate as the precipitant. X-ray diffraction data were collected to 2.2 A resolution using Cu K alpha radiation. The crystal belongs to the orthorhombic space group P2(1)2(1)2(1), with unit-cell parameters a = 69.90, b = 70.86 A, c = 75.55 A. Assuming the presence of a trimer in the asymmetric unit, the solvent content was 30%, with a V(M) of 1.8 A3 Da(-1).
 ==About this Structure==
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 [[Category: magnesium]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Feb 13 08:20:15 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:28:07 2008''