2cbj: Difference between revisions

Revision as of 17:46, 21 February 2008

STRUCTURE OF THE CLOSTRIDIUM PERFRINGENS NAGJ FAMILY 84 GLYCOSIDE HYDROLASE, A HOMOLOGUE OF HUMAN O-GLCNACASE IN COMPLEX WITH PUGNAC

OverviewOverview

O-linked N-acetylglucosamine (O-GlcNAc) modification of specific serines/threonines on intracellular proteins in higher eukaryotes has been shown to directly regulate important processes such as the cell cycle, insulin sensitivity and transcription. The structure, molecular mechanisms of catalysis, protein substrate recognition/specificity of the eukaryotic O-GlcNAc transferase and hydrolase are largely unknown. Here we describe the crystal structure, enzymology and in vitro activity on human substrates of Clostridium perfringens NagJ, a close homologue of human O-GlcNAcase (OGA), representing the first family 84 glycoside hydrolase structure. The structure reveals a deep active site pocket highly conserved with the human enzyme, compatible with binding of O-GlcNAcylated peptides. Together with mutagenesis data, the structure supports a variant of the substrate-assisted catalytic mechanism, involving two aspartic acids and an unusually positioned tyrosine. Insights into recognition of substrate come from a complex with the transition state mimic O-(2-acetamido-2-deoxy-D-glucopyranosylidene)amino-N-phenylcarbamate (Ki=5.4 nM). Strikingly, the enzyme is inhibited by the pseudosubstrate peptide Ala-Cys(-S-GlcNAc)-Ala, and has OGA activity against O-GlcNAcylated human proteins, suggesting that the enzyme is a suitable model for further studies into the function of human OGA.

About this StructureAbout this Structure

2CBJ is a Single protein structure of sequence from Clostridium perfringens with and as ligands. Active as Hyalurononglucosaminidase, with EC number 3.2.1.35 Known structural/functional Site: . Full crystallographic information is available from OCA.

ReferenceReference

Structural insights into the mechanism and inhibition of eukaryotic O-GlcNAc hydrolysis., Rao FV, Dorfmueller HC, Villa F, Allwood M, Eggleston IM, van Aalten DM, EMBO J. 2006 Apr 5;25(7):1569-78. Epub 2006 Mar 16. PMID:16541109

Page seeded by OCA on Thu Feb 21 16:46:57 2008

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 ==Overview==
-O-linked N-acetylglucosamine (O-GlcNAc) modification of specific, serines/threonines on intracellular proteins in higher eukaryotes has been, shown to directly regulate important processes such as the cell cycle, insulin sensitivity and transcription. The structure, molecular mechanisms, of catalysis, protein substrate recognition/specificity of the eukaryotic, O-GlcNAc transferase and hydrolase are largely unknown. Here we describe, the crystal structure, enzymology and in vitro activity on human, substrates of Clostridium perfringens NagJ, a close homologue of human, O-GlcNAcase (OGA), representing the first family 84 glycoside hydrolase, structure. The structure reveals a deep active site pocket highly, conserved with the human enzyme, compatible with binding of O-GlcNAcylated, peptides. Together with mutagenesis data, the structure supports a variant, of the substrate-assisted catalytic mechanism, involving two aspartic, acids and an unusually positioned tyrosine. Insights into recognition of, substrate come from a complex with the transition state mimic, O-(2-acetamido-2-deoxy-D-glucopyranosylidene)amino-N-phenylcarbamate, (Ki=5.4 nM). Strikingly, the enzyme is inhibited by the pseudosubstrate, peptide Ala-Cys(-S-GlcNAc)-Ala, and has OGA activity against, O-GlcNAcylated human proteins, suggesting that the enzyme is a suitable, model for further studies into the function of human OGA.
+O-linked N-acetylglucosamine (O-GlcNAc) modification of specific serines/threonines on intracellular proteins in higher eukaryotes has been shown to directly regulate important processes such as the cell cycle, insulin sensitivity and transcription. The structure, molecular mechanisms of catalysis, protein substrate recognition/specificity of the eukaryotic O-GlcNAc transferase and hydrolase are largely unknown. Here we describe the crystal structure, enzymology and in vitro activity on human substrates of Clostridium perfringens NagJ, a close homologue of human O-GlcNAcase (OGA), representing the first family 84 glycoside hydrolase structure. The structure reveals a deep active site pocket highly conserved with the human enzyme, compatible with binding of O-GlcNAcylated peptides. Together with mutagenesis data, the structure supports a variant of the substrate-assisted catalytic mechanism, involving two aspartic acids and an unusually positioned tyrosine. Insights into recognition of substrate come from a complex with the transition state mimic O-(2-acetamido-2-deoxy-D-glucopyranosylidene)amino-N-phenylcarbamate (Ki=5.4 nM). Strikingly, the enzyme is inhibited by the pseudosubstrate peptide Ala-Cys(-S-GlcNAc)-Ala, and has OGA activity against O-GlcNAcylated human proteins, suggesting that the enzyme is a suitable model for further studies into the function of human OGA.
 ==About this Structure==
@@ Line 14: / Line 14: @@
 [[Category: Hyalurononglucosaminidase]]
 [[Category: Single protein]]
-[[Category: Aalten, D.M.F.Van.]]
+[[Category: Aalten, D M.F Van.]]
 [[Category: Allwood, M.]]
-[[Category: Dorfmueller, H.C.]]
+[[Category: Dorfmueller, H C.]]
-[[Category: Eggleston, I.M.]]
+[[Category: Eggleston, I M.]]
-[[Category: Rao, F.V.]]
+[[Category: Rao, F V.]]
 [[Category: Villa, F.]]
 [[Category: CL]]
@@ Line 29: / Line 29: @@
 [[Category: o-glcnac]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Feb  3 10:33:39 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:46:57 2008''