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[[Image:3a2y.png|left|200px]]
==E. coli Gsp amidase C59A complexed with Gsp==
<StructureSection load='3a2y' size='340' side='right' caption='[[3a2y]], [[Resolution|resolution]] 1.95&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[3a2y]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3A2Y OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3A2Y FirstGlance]. <br>
</td></tr><tr><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=TS5:GLUTATHIONYLSPERMIDINE'>TS5</scene><br>
<tr><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2iob|2iob]], [[3a2z|3a2z]], [[3a30|3a30]]</td></tr>
<tr><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">gsp, b2988, JW2956 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])</td></tr>
<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3a2y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3a2y OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3a2y RCSB], [http://www.ebi.ac.uk/pdbsum/3a2y PDBsum]</span></td></tr>
<table>
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/a2/3a2y_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The bifunctional Escherichia coli glutathionylspermidine synthetase/amidase (GspSA), catalyzes both the synthesis and hydrolysis of Gsp. Its amidase domain (GspA), which catalyzes the hydrolysis of Gsp into glutathione and spermidine, plays an important role in redox sensing and protein S-thiolation. To gain insight of the regulation and catalytic mechanism of GspA and further understand the recycling of the Gsp dimer and Gsp-S-protein adducts, we solved two crystal structures of GspA and GspSA both with the C59A mutation and bound with the substrate, Gsp. In both structures, Cys59, His131 and Glu147 form the catalytic triad, which is similar to other cysteine proteases. Comparison of the GspA_Gsp complex and apo GspSA structures indicates that upon binding with Gsp, the side chains of Asn149 and Gln58 of the amidase domain are induced to move closer to the carbonyl oxygen of the cleaved amide bond of Gsp, thereby participating in catalysis. In addition, the helix-loop region of GspA, corresponding to the sequence (30)YSSLDPQEYEDDA(42), involves in regulating the substrate binding. Our previous study indicated that the thiol of Cys59 of GspA is only oxidized to sulfenic acid by H(2)O(2). When comparing the active site of GspA with those of other cysteine proteases, we found that limited space and hydrophobicity of the environment around Cys59 play an important role to inhibit its further oxidation. The structural results presented here not only elucidate the catalytic mechanism and regulation of GspA, but also help us design small molecules to inhibit or probe for the activity of GspA.


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Structure and mechanism of Escherichia coli glutathionylspermidine amidase belonging to the family of cysteine, histidine-dependent amidohydrolases/peptidases.,Pai CH, Wu HJ, Lin CH, Wang AH Protein Sci. 2011 Jan 11. PMID:21226054<ref>PMID:21226054</ref>
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{{STRUCTURE_3a2y|  PDB=3a2y  |  SCENE=  }}


===E. coli Gsp amidase C59A complexed with Gsp===
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
 
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== References ==
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The line below this paragraph, {{ABSTRACT_PUBMED_21226054}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 21226054 is the PubMed ID number.
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{{ABSTRACT_PUBMED_21226054}}
 
==About this Structure==
[[3a2y]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3A2Y OCA].
 
==Reference==
<ref group="xtra">PMID:021226054</ref><references group="xtra"/>
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
[[Category: Chiang, B Y.]]
[[Category: Chiang, B Y.]]

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