RNase A NMR: Difference between revisions
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<scene name='Sandbox_Reserved_199/2aas_-_five_amide_backbone_pro/3'>Five backbone amide protons</scene> were shown to be involved in folding-related intermolecular interactions during initial protein folding steps: amide protons from <scene name='Sandbox_Reserved_199/2aas_-_val_63/2'>Val 63</scene>, <scene name='Sandbox_Reserved_199/2aas_-_ile81/2'>Ile 81</scene>, <scene name='Sandbox_Reserved_199/2aas_-_thr82/1'>Thr 82</scene>, <scene name='Sandbox_Reserved_199/2aas_-_ile106/1'>Ile 106</scene>, and <scene name='Sandbox_Reserved_199/2aas_-_val_118/1'>Val 118</scene>. All five of these protons are involved in hydrogen bonding within the <scene name='Sandbox_Reserved_199/2aas_-_beta_sheet/2'>β sheet secondary structure</scene> of Ribonuclease; therefore, β sheets were proposed to be the starting point for the folding mechanism of Ribonuclease. Furthermore, this supports the formation of a stable secondary structure before the formation of the <scene name='Sandbox_Reserved_199/2aas_-_final_tertiary_structue/1'>final tertiary structure</scene>, which is consistent with the framework model of [http://en.wikipedia.org/wiki/Protein_folding#Protein_nuclear_magnetic_resonance_spectroscopy protein folding mechanisms]. | <scene name='Sandbox_Reserved_199/2aas_-_five_amide_backbone_pro/3'>Five backbone amide protons</scene> were shown to be involved in folding-related intermolecular interactions during initial protein folding steps: amide protons from <scene name='Sandbox_Reserved_199/2aas_-_val_63/2'>Val 63</scene>, <scene name='Sandbox_Reserved_199/2aas_-_ile81/2'>Ile 81</scene>, <scene name='Sandbox_Reserved_199/2aas_-_thr82/1'>Thr 82</scene>, <scene name='Sandbox_Reserved_199/2aas_-_ile106/1'>Ile 106</scene>, and <scene name='Sandbox_Reserved_199/2aas_-_val_118/1'>Val 118</scene>. All five of these protons are involved in hydrogen bonding within the <scene name='Sandbox_Reserved_199/2aas_-_beta_sheet/2'>β sheet secondary structure</scene> of Ribonuclease; therefore, β sheets were proposed to be the starting point for the folding mechanism of Ribonuclease. Furthermore, this supports the formation of a stable secondary structure before the formation of the <scene name='Sandbox_Reserved_199/2aas_-_final_tertiary_structue/1'>final tertiary structure</scene>, which is consistent with the framework model of [http://en.wikipedia.org/wiki/Protein_folding#Protein_nuclear_magnetic_resonance_spectroscopy protein folding mechanisms]. | ||
<Structure load='2AAS' size='350' frame='true' align='right' caption='2AAS - NMR Scructure of Bovine Pancreatic Ribonuclease' scene='Sandbox_Reserved_199/2aas_-_all_models/4' /> | <Structure load='2AAS' size='350' frame='true' align='right' caption='2AAS - NMR Scructure of Bovine Pancreatic Ribonuclease, [[2aas]]' scene='Sandbox_Reserved_199/2aas_-_all_models/4' /> | ||
==Ribonuclease NMR Structure Versus X-Ray Crystallography Ribonuclease Structure<ref name="Santoro">PMID: 8381876 </ref>== | ==Ribonuclease NMR Structure Versus X-Ray Crystallography Ribonuclease Structure<ref name="Santoro">PMID: 8381876 </ref>== | ||
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==Solution Structure and Dynamics of Human Pancreatic Ribonuclease<ref name="rico">PMID: 18495155</ref>== | ==Solution Structure and Dynamics of Human Pancreatic Ribonuclease<ref name="rico">PMID: 18495155</ref>== | ||
<Structure load='2k11' size='350' frame='true' align='right' caption=' | <Structure load='2k11' size='350' frame='true' align='right' caption='[[2k11]] - NMR Structure of Human Pancreatic Ribonuclese' scene='Sandbox_Reserved_199/2k11_all_models/2' /> | ||
===Experimental Procedure=== | ===Experimental Procedure=== | ||
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* [http://www.proteopedia.org/wiki/index.php/RNaseS_RNaseB RNase S and RNase B] | * [http://www.proteopedia.org/wiki/index.php/RNaseS_RNaseB RNase S and RNase B] | ||
* [http://www.proteopedia.org/wiki/index.php/RNaseA_Nobel_Prizes RNase A Nobel Prizes] | * [http://www.proteopedia.org/wiki/index.php/RNaseA_Nobel_Prizes RNase A Nobel Prizes] | ||
==3D structures of ribonuclease== | |||
[[Ribonuclease]] | |||
== References == | == References == | ||