Met repressor sandbox (Beasley): Difference between revisions
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===''E. coli'' met repressor=== | ===''E. coli'' met repressor=== | ||
The <scene name='Met_repressor_(Beasley)/Met_repressor_only/1'>met repressor</scene> regulates the transcription genes involved in the biosythesis of methionine in E. coli, and is the product of the metJ gene <ref name="Voet">Voet, Donald, Judith G. Voet, and Charlotte W. Pratt. Fundamentals of Biochemistry Life at the Molecular Level. New York: John Wiley & Sons, 2008. p. 578-579. Print.</ref> Methionine is an important amino acid that acts as the initiator of protein synthesis (as N-formyl methionine) and of protein elongation. It is also the precursor of spermidine, a polyamine involved in cellular metabolism.<ref name="Phillips,S">PMID 8735275</ref> The met repressor is a dimer of identical 104 amino acid subunits, and is capable of repressing or depressing target genes within 30 minutes of a change of methionine concentration.<ref name="Augustus">PMID 19289840</ref> Each subunit contains a flexible loop (residues 12-20) leading into a β-strand that pairs with the related strand of the other subunit to form a two stranded antiparallel β-sheet or β-ribbon.<ref name=Phillips,S" /> The rest of the met repressor subunit consists of three α helices: A (30-45), B (52-66), C (86-94) linked by different sized loops.<ref name=Phillips,S" /> | The <scene name='Met_repressor_(Beasley)/Met_repressor_only/1'>met repressor</scene> regulates the transcription genes involved in the biosythesis of methionine in E. coli, and is the product of the metJ gene <ref name="Voet">Voet, Donald, Judith G. Voet, and Charlotte W. Pratt. Fundamentals of Biochemistry Life at the Molecular Level. New York: John Wiley & Sons, 2008. p. 578-579. Print.</ref> Methionine is an important amino acid that acts as the initiator of protein synthesis (as N-formyl methionine) and of protein elongation. It is also the precursor of spermidine, a polyamine involved in cellular metabolism.<ref name="Phillips,S">PMID 8735275</ref> The met repressor is a dimer of identical 104 amino acid subunits, and is capable of repressing or depressing target genes within 30 minutes of a change of methionine concentration.<ref name="Augustus">PMID 19289840</ref> Each subunit contains a flexible loop (residues 12-20) leading into a β-strand that pairs with the related strand of the other subunit to form a two stranded antiparallel β-sheet or β-ribbon.<ref name="Phillips,S" /> The rest of the met repressor subunit consists of three α helices: A (30-45), B (52-66), C (86-94) linked by different sized loops.<ref name="Phillips,S" /> | ||
==Key structural features of the met repressor== | ==Key structural features of the met repressor== | ||