Myosin: Difference between revisions
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{{STRUCTURE_2mys| PDB=2mys | SIZE=300| SCENE=Myosin/Cv/1 |right|CAPTION=Chicken myosin with di-methylated lysines complex with sulfate and Mg+2 ion [[2mys]] }} | {{STRUCTURE_2mys| PDB=2mys | SIZE=300| SCENE=Myosin/Cv/1 |right|CAPTION=Chicken myosin with di-methylated lysines complex with sulfate and Mg+2 ion [[2mys]] }} | ||
[[Myosin]] is one of three major classes of molecular motor proteins: myosin, dynein, and kinesin. As the most abundant of these proteins myosin plays a structural and enzymatic role in muscle contraction and intracellular motility. Myosin was first discovered in muscle in the 19th century. <ref name="Spudich">PMID: 8824453 </ref> Myosin is a superfamily of proteins which bind actin, hydrolyze ATP and transduce force. Thus most are located in muscle cells. Composed of head, neck and tail domains. Head domain binds the actin and moves along it. The neck is a linker and binds the light chains which have a regulatory function. The tail interacts with cargo molecules (CBD)m. There are 18 classes of myosin. Myosin II (MII) is best studied and contains 2 heavy chains (HC) which constitute the head and tail | [[Myosin]] is one of three major classes of molecular motor proteins: myosin, dynein, and kinesin. As the most abundant of these proteins myosin plays a structural and enzymatic role in muscle contraction and intracellular motility. Myosin was first discovered in muscle in the 19th century. <ref name="Spudich">PMID: 8824453 </ref> Myosin is a superfamily of proteins which bind actin, hydrolyze ATP and transduce force. Thus most are located in muscle cells. Composed of head, neck and tail domains. Head domain binds the actin and moves along it. The neck is a linker and binds the light chains which have a regulatory function. The tail interacts with cargo molecules (CBD)m. There are 18 classes of myosin. Myosin II (MII) is best studied and contains 2 heavy chains (HC) which constitute the head or motor domain (MD) and the tail domain and 4 light chains (LC) which are referred to as the essential LC (ELC) and the regulatory LC (RLC). The images at the left and at the right correspond to one representative Myosin, ''i.e.'' the crystal structure of from Myosin ''Gallus gallus'' ([[2mys]]). | ||
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[[1lkx]] – DdMI HC - ''Dictyostelium discoideum''<br /> | [[1lkx]] – DdMI HC - ''Dictyostelium discoideum''<br /> | ||
[[2drk]], [[2drm]] – MI HC + 10-mer peptide – ''Acanthamoeba castellanii'' | [[2drk]], [[2drm]] – MI HC + 10-mer peptide – ''Acanthamoeba castellanii''<br /> | ||
[[2xmf]] – mMI SH3 domain - mouse | |||
=== Myosin II === | === Myosin II === | ||
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[[3i5i]] – LpMII RLC+HC+ELC+SO4<br /> | [[3i5i]] – LpMII RLC+HC+ELC+SO4<br /> | ||
[[2jhr]] – DdMII HC+ADP-VO4+pentabromopseudilin<br /> | [[2jhr]] – DdMII HC+ADP-VO4+pentabromopseudilin<br /> | ||
[[2xo8]] - DdMII HC | [[2xo8]] - DdMII HC MD +pseudilin derivative<br /> | ||
[[2y0r]], [[2y8i]], [[2y9e]], [[3myk]] - DdMII HC MD (mutant)<br /> | |||
[[3bz7]], [[3bz9]], [[1iv3]], [[3bz8]] - DdMII HC+blebbistatin<br /> | [[3bz7]], [[3bz9]], [[1iv3]], [[3bz8]] - DdMII HC+blebbistatin<br /> | ||
[[3mjx]] - DdMII HC+blebbistatin + ADP-VO4<br /> | [[3mjx]] - DdMII HC+blebbistatin + ADP-VO4<br /> | ||
[[3mnq]] - DdMII HC | [[3mnq]] - DdMII HC MD + ADP-VO4 + reservatrol<br /> | ||
[[3bas]], [[3bat]], [[1fmv]] - DdMII HC<br /> | [[3bas]], [[3bat]], [[1fmv]] - DdMII HC<br /> | ||
[[1fmw]] – DdMII HC+ATP<br /> | [[1fmw]] – DdMII HC+ATP<br /> | ||
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[[1g8x]] – DdMII+actinin 3<br /> | [[1g8x]] – DdMII+actinin 3<br /> | ||
[[2jj9]], [[2x9h]] - DdMII HC+ADP-VO4<br /> | [[2jj9]], [[2x9h]] - DdMII HC+ADP-VO4<br /> | ||
[[3mkd]] - DdMII HC | [[3mkd]] - DdMII HC MD (mutant) + ADP-VO4 <br /> | ||
[[1lvk]], [[1mma]], [[1mmg]], [[1mmn]] – DdMII HC (mutant)+Mg+nucleotide<br /> | [[1lvk]], [[1mma]], [[1mmg]], [[1mmn]] – DdMII HC (mutant)+Mg+nucleotide<br /> | ||
[[1myh]], [[1myk]], [[1myl]], [[3myh]], [[3myl]] - DdMII HC (mutant)<br /> | [[1myh]], [[1myk]], [[1myl]], [[3myh]], [[3myl]] - DdMII HC (mutant)<br /> | ||
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[[1mnd]], [[1w9j]], [[1w9l]] - DdMII HC (mutant)+Mg+ADP+AlF4<br /> | [[1mnd]], [[1w9j]], [[1w9l]] - DdMII HC (mutant)+Mg+ADP+AlF4<br /> | ||
[[2aka]] - DdMII HC+dynamin-1<br /> | [[2aka]] - DdMII HC+dynamin-1<br /> | ||
[[2xel]] – DdMII HC + inhibitor<br /> | |||
[[1n2d]] – ScMII LC+IQ2 IQ3 motifs from Myo2p<br /> | [[1n2d]] – ScMII LC+IQ2 IQ3 motifs from Myo2p<br /> | ||
[[1m45]] - ScMII LC+IQ2 motif from Myo2p<br /> | [[1m45]] - ScMII LC+IQ2 motif from Myo2p<br /> | ||
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===Myosin X=== | ===Myosin X=== | ||
[[3pzd]] - hMX myth4-ferm tandem + netrin receptor DCC | [[3aux]] - hMX myth4-ferm tandem<br /> | ||
[[3pzd]], [[3au4]] - hMX myth4-ferm tandem + netrin receptor DCC | |||
===Flight muscle myosin=== | ===Flight muscle myosin=== | ||