Shiga toxin: Difference between revisions

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Max Evoy-Mount, Michal Harel, Alexander Berchansky

Revision as of 05:36, 7 November 2011 view source Max Evoy-Mount (talk \| contribs) 32 edits No edit summary ← Older edit		Revision as of 05:56, 7 November 2011 view source Max Evoy-Mount (talk \| contribs) 32 edits No edit summary Newer edit →
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	==Function==		==Function==

	Shiga Toxin acts as an N-glycosidase, removing an adenine from the 60S ribosomal rRNA of a target cell leading to reduced protein synthesis.<ref name=Di>PMID: 21184769</ref> The B subunit is necessary for binding to globo series glycolipid globotriaosylceramide (Gb3), a eukaryotic membrane receptor, where it is then endocytosed and proteolytically cleaved into two active A subunits and a B subunit.<ref name=Lenz>PMID: 2170899</ref> Once the A subunit is transported to the cytosol it acts by depurinating the 28S ribosomal RNA which leads to inhibition of protein elongation and ultimately cellular apoptosis. On the A subunit <scene name='~~Shiga_toxin~~/~~Active_site~~/1'>Tyr77, Tyr114, Glu167, Arg170, and Trp203</scene> are all essential in glycosidic activity.<ref name=Di>PMID: 21184769</ref>		Shiga Toxin acts as an N-glycosidase, removing an adenine from the 60S ribosomal rRNA of a target cell leading to reduced protein synthesis.<ref name=Di>PMID: 21184769</ref> The B subunit is necessary for binding to globo series glycolipid globotriaosylceramide (Gb3), a eukaryotic membrane receptor, where it is then endocytosed and proteolytically cleaved into two active A subunits and a B subunit.<ref name=Lenz>PMID: 2170899</ref> Once the A subunit is transported to the cytosol it acts by depurinating the 28S ribosomal RNA which leads to inhibition of protein elongation and ultimately cellular apoptosis. On the A subunit <scene name='Shiga_toxin_1/Active_site_zoomed_in/1'>Tyr77, Tyr114, Glu167, Arg170, and Trp203</scene> are all essential in glycosidic activity.<ref name=Di>PMID: 21184769</ref>