3qs4: Difference between revisions

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-[[Image:3qs4.png|left|200px]]
+==Crystal structure of LeuT mutant F259V bound to sodium and L-tryptophan==
+<StructureSection load='3qs4' size='340' side='right' caption='[[3qs4]], [[Resolution|resolution]] 2.63&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[3qs4]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Aquifex_aeolicus Aquifex aeolicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3QS4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3QS4 FirstGlance]. <br>
+</td></tr><tr><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BOG:B-OCTYLGLUCOSIDE'>BOG</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=TRP:TRYPTOPHAN'>TRP</scene><br>
+<tr><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2a65|2a65]], [[3f3a|3f3a]], [[3qs5|3qs5]], [[3qs6|3qs6]]</td></tr>
+<tr><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">aq_2077, leut, snf ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=63363 Aquifex aeolicus])</td></tr>
+<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3qs4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3qs4 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3qs4 RCSB], [http://www.ebi.ac.uk/pdbsum/3qs4 PDBsum]</span></td></tr>
+<table>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+LeuT is a bacterial homologue of the neurotransmitter:sodium symporter (NSS) family and, being the only NSS member to have been structurally characterized by X-ray crystallography, is a model protein for studying transporter structure and mechanism. Transport activity in LeuT was hypothesized to require structural transitions between open-to-out and occluded conformations dependent upon protein:ligand binding complementarity. Here, using crystallographic and functional analysis, we show that binding site modification produces changes in both structure and activity that are consistent with complementarity-dependent structural transitions to the occluded state. The mutation I359Q converts the activity of tryptophan from inhibitor to transportable substrate. This mutation changes the local environment of the binding site, inducing the bound tryptophan to adopt a different conformer than in the wild-type complex. Instead of trapping the transporter open, tryptophan binding now allows the formation of an occluded state. Thus, transport activity is correlated to the ability of the ligand to promote the structural transition to the occluded state, a step in the transport cycle that is dependent on protein:ligand complementarity in the central binding site.
-<!--
+Insights into transport mechanism from LeuT engineered to transport tryptophan.,Piscitelli CL, Gouaux E EMBO J. 2011 Sep 27. doi: 10.1038/emboj.2011.353. PMID:21952050<ref>PMID:21952050</ref>
-The line below this paragraph, containing "STRUCTURE_3qs4", creates the "Structure Box" on the page.
-You may change the PDB parameter (which sets the PDB file loaded into the applet)
-or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-or leave the SCENE parameter empty for the default display.
--->
-{{STRUCTURE_3qs4|  PDB=3qs4  |  SCENE=  }}
-===Crystal structure of LeuT mutant F259V bound to sodium and L-tryptophan===
+From MEDLINEÂ®/PubMedÂ®, a database of the U.S. National Library of Medicine.<br>
+</div>
+==See Also==
-<!--
+*[[Leucine transporter|Leucine transporter]]
-The line below this paragraph, {{ABSTRACT_PUBMED_21952050}}, adds the Publication Abstract to the page
+== References ==
-(as it appears on PubMed at http://www.pubmed.gov), where 21952050 is the PubMed ID number.
+<references/>
--->
+__TOC__
-{{ABSTRACT_PUBMED_21952050}}
+</StructureSection>
-==About this Structure==
-[[3qs4]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Aquifex_aeolicus Aquifex aeolicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3QS4 OCA].
-==Reference==
-<ref group="xtra">PMID:021952050</ref><references group="xtra"/>
 [[Category: Aquifex aeolicus]]
 [[Category: Gouaux, E.]]