1h58: Difference between revisions
No edit summary |
No edit summary |
||
| Line 4: | Line 4: | ||
==Overview== | ==Overview== | ||
A molecular description of oxygen and peroxide activation in biological | A molecular description of oxygen and peroxide activation in biological systems is difficult, because electrons liberated during X-ray data collection reduce the active centres of redox enzymes catalysing these reactions. Here we describe an effective strategy to obtain crystal structures for high-valency redox intermediates and present a three-dimensional movie of the X-ray-driven catalytic reduction of a bound dioxygen species in horseradish peroxidase (HRP). We also describe separate experiments in which high-resolution structures could be obtained for all five oxidation states of HRP, showing such structures with preserved redox states for the first time. | ||
==About this Structure== | ==About this Structure== | ||
| Line 14: | Line 14: | ||
[[Category: Peroxidase]] | [[Category: Peroxidase]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Berglund, G | [[Category: Berglund, G I.]] | ||
[[Category: Carlsson, G | [[Category: Carlsson, G H.]] | ||
[[Category: Hajdu, J.]] | [[Category: Hajdu, J.]] | ||
[[Category: Henriksen, A.]] | [[Category: Henriksen, A.]] | ||
[[Category: Smith, A | [[Category: Smith, A T.]] | ||
[[Category: Szoke, H.]] | [[Category: Szoke, H.]] | ||
[[Category: ACT]] | [[Category: ACT]] | ||
| Line 28: | Line 28: | ||
[[Category: peroxidase]] | [[Category: peroxidase]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:57:30 2008'' | ||
Revision as of 13:57, 21 February 2008
|
STRUCTURE OF FERROUS HORSERADISH PEROXIDASE C1A
OverviewOverview
A molecular description of oxygen and peroxide activation in biological systems is difficult, because electrons liberated during X-ray data collection reduce the active centres of redox enzymes catalysing these reactions. Here we describe an effective strategy to obtain crystal structures for high-valency redox intermediates and present a three-dimensional movie of the X-ray-driven catalytic reduction of a bound dioxygen species in horseradish peroxidase (HRP). We also describe separate experiments in which high-resolution structures could be obtained for all five oxidation states of HRP, showing such structures with preserved redox states for the first time.
About this StructureAbout this Structure
1H58 is a Single protein structure of sequence from Armoracia rusticana with , and as ligands. Active as Peroxidase, with EC number 1.11.1.7 Known structural/functional Site: . Full crystallographic information is available from OCA.
ReferenceReference
The catalytic pathway of horseradish peroxidase at high resolution., Berglund GI, Carlsson GH, Smith AT, Szoke H, Henriksen A, Hajdu J, Nature. 2002 May 23;417(6887):463-8. PMID:12024218
Page seeded by OCA on Thu Feb 21 12:57:30 2008