2x61: Difference between revisions

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-[[Image:2x61.png|left|200px]]
+==CRYSTAL STRUCTURE OF THE SIALYLTRANSFERASE CST-II IN COMPLEX WITH TRISACCHARIDE ACCEPTOR AND CMP==
+<StructureSection load='2x61' size='340' side='right' caption='[[2x61]], [[Resolution|resolution]] 1.95&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[2x61]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Campylobacter_jejuni Campylobacter jejuni]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2X61 OCA]. <br>
+</td></tr><tr><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CH:N3-PROTONATED+CYTIDINE-5-MONOPHOSPHATE'>CH</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene>, <scene name='pdbligand=NGA:N-ACETYL-D-GALACTOSAMINE'>NGA</scene><br>
+<tr><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=SIA:O-SIALIC+ACID'>SIA</scene></td></tr>
+<tr><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1ro8|1ro8]], [[2x63|2x63]], [[2x62|2x62]], [[1ro7|1ro7]]</td></tr>
+<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glucokinase Glucokinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.2 2.7.1.2] </span></td></tr>
+<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2x61 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2x61 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2x61 RCSB], [http://www.ebi.ac.uk/pdbsum/2x61 PDBsum]</span></td></tr>
+<table>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Sialic acids play important roles in various biological processes and typically terminate the oligosaccharide chains on the cell surfaces of a wide range of organisms including mammals and bacteria. Their attachment is catalyzed by a set of sialyltransferases with defined specificities both for their acceptor sugars and the position of attachment. However, little is known of how this specificity is encoded. The structure of the bifunctional sialyltransferase Cst-II of the human pathogen Campylobacter jejuni in complex with CMP and the terminal trisaccharide of its natural acceptor (Neu5Ac-alpha-2,3-Gal-beta-1,3-GalNAc) has been solved at 1.95 A resolution and its kinetic mechanism shown to be iso-ordered Bi Bi, consistent with its dual acceptor substrate specificity. The trisaccharide acceptor is seen to bind to the active site of Cst-II through interactions primarily mediated by Asn-51, Tyr-81, and Arg-129. Kinetic and structural analyses of mutants modified at these positions indicate that these residues are critical for acceptor binding and catalysis, thereby providing significant new insight into the kinetic and catalytic mechanism and acceptor specificity of this pathogen encoded bifunctional GT-42 sialyltransferase.
-<!--
+Structural and kinetic analysis of substrate binding to the sialyltransferase CST-II from Campylobacter Jejuni.,Lee HJ, Lairson LL, Rich JR, Lameignere E, Wakarchuk WW, Withers SG, Strynadka NC J Biol Chem. 2011 Aug 8. PMID:21832050<ref>PMID:21832050</ref>
-The line below this paragraph, containing "STRUCTURE_2x61", creates the "Structure Box" on the page.
-You may change the PDB parameter (which sets the PDB file loaded into the applet)
-or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-or leave the SCENE parameter empty for the default display.
--->
-{{STRUCTURE_2x61|  PDB=2x61  |  SCENE=  }}
-===CRYSTAL STRUCTURE OF THE SIALYLTRANSFERASE CST-II IN COMPLEX WITH TRISACCHARIDE ACCEPTOR AND CMP===
+From MEDLINEÂ®/PubMedÂ®, a database of the U.S. National Library of Medicine.<br>
+</div>
+== References ==
-<!--
+<references/>
-The line below this paragraph, {{ABSTRACT_PUBMED_21832050}}, adds the Publication Abstract to the page
+__TOC__
-(as it appears on PubMed at http://www.pubmed.gov), where 21832050 is the PubMed ID number.
+</StructureSection>
--->
-{{ABSTRACT_PUBMED_21832050}}
-==About this Structure==
-[[2x61]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Campylobacter_jejuni Campylobacter jejuni]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2X61 OCA].
-==Reference==
-<ref group="xtra">PMID:021832050</ref><references group="xtra"/>
 [[Category: Campylobacter jejuni]]
 [[Category: Lairson, L L.]]