1e9m: Difference between revisions

Revision as of 13:25, 21 February 2008

FERREDOXIN VI FROM RHODOBACTER CAPSULATUS

OverviewOverview

A [2Fe-2S] ferredoxin found in the photosynthetic bacterium Rhodobacter capsulatus has been purified in recombinant form from Escherichia coli. This protein, called FdVI, resembles ferredoxins involved in iron-sulfur cluster biosynthesis in various prokaryotic and eukaryotic cells. Purified recombinant FdVI was recovered in high yields and appeared to be indistinguishable from the genuine R. capsulatus ferredoxin based on UV-visible absorption and EPR spectroscopy and mass spectrometry. FdVI has been crystallized in the oxidized state by a sitting-drop vapour-diffusion technique using sodium formate as precipitant. Seeding larger drops from a previous hanging-drop-grown small crystal resulted in the formation of long red-brown prismatic needles. Preliminary X-ray diffraction analysis indicated that FdVI crystals are orthorhombic and belong to the space group P2(1)2(1)2(1), with unit-cell parameters a = 45.87, b = 49.83, c = 54.29 A.

About this StructureAbout this Structure

1E9M is a Single protein structure of sequence from Rhodobacter capsulatus with as ligand. Known structural/functional Site: . Full crystallographic information is available from OCA.

ReferenceReference

Crystallization and preliminary X-ray diffraction analysis of a [2Fe-2S] ferredoxin (FdVI) from Rhodobacter capsulatus., Armengaud J, Sainz G, Jouanneau Y, Sieker LC, Acta Crystallogr D Biol Crystallogr. 2001 Feb;57(Pt 2):301-3. PMID:11173487 [[Category: [2fe-2s]]]

Page seeded by OCA on Thu Feb 21 12:25:22 2008

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OCA

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 ==Overview==
-A [2Fe-2S] ferredoxin found in the photosynthetic bacterium Rhodobacter, capsulatus has been purified in recombinant form from Escherichia coli., This protein, called FdVI, resembles ferredoxins involved in iron-sulfur, cluster biosynthesis in various prokaryotic and eukaryotic cells. Purified, recombinant FdVI was recovered in high yields and appeared to be, indistinguishable from the genuine R. capsulatus ferredoxin based on, UV-visible absorption and EPR spectroscopy and mass spectrometry. FdVI has, been crystallized in the oxidized state by a sitting-drop vapour-diffusion, technique using sodium formate as precipitant. Seeding larger drops from a, previous hanging-drop-grown small crystal resulted in the formation of, long red-brown prismatic needles. Preliminary X-ray diffraction analysis, indicated that FdVI crystals are orthorhombic and belong to the space, group P2(1)2(1)2(1), with unit-cell parameters a = 45.87, b = 49.83, c =, 54.29 A.
+A [2Fe-2S] ferredoxin found in the photosynthetic bacterium Rhodobacter capsulatus has been purified in recombinant form from Escherichia coli. This protein, called FdVI, resembles ferredoxins involved in iron-sulfur cluster biosynthesis in various prokaryotic and eukaryotic cells. Purified recombinant FdVI was recovered in high yields and appeared to be indistinguishable from the genuine R. capsulatus ferredoxin based on UV-visible absorption and EPR spectroscopy and mass spectrometry. FdVI has been crystallized in the oxidized state by a sitting-drop vapour-diffusion technique using sodium formate as precipitant. Seeding larger drops from a previous hanging-drop-grown small crystal resulted in the formation of long red-brown prismatic needles. Preliminary X-ray diffraction analysis indicated that FdVI crystals are orthorhombic and belong to the space group P2(1)2(1)2(1), with unit-cell parameters a = 45.87, b = 49.83, c = 54.29 A.
 ==About this Structure==
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 [[Category: ferredoxin]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Feb  3 09:38:32 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:25:22 2008''