1bhn: Difference between revisions

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NUCLEOSIDE DIPHOSPHATE KINASE ISOFORM A FROM BOVINE RETINA

OverviewOverview

The crystal structures of two isoforms of nucleoside diphosphate kinase from bovine retina overexpressed in Escherischia coli have been determined to 2.4 A resolution. Both the isoforms, NBR-A and NBR-B, are hexameric and the fold of the monomer is in agreement with NDP-kinase structures from other biological sources. Although the polypeptide chains of the two isoforms differ by only two residues, they crystallize in different space groups. NBR-A crystallizes in space group P212121 with an entire hexamer in the asymmetric unit, while NBR-B crystallizes in space group P43212 with a trimer in the asymmetric unit. The highly conserved nucleotide-binding site observed in other nucleoside diphosphate kinase structures is also observed here. Both NBR-A and NBR-B were crystallized in the presence of cGMP. The nucleotide is bound with the base in the anti conformation. The NBR-A active site contained both cGMP and GDP each bound at half occupancy. Presumably, NBR-A had retained GDP (or GTP) from the purification process. The NBR-B active site contained only cGMP.

About this StructureAbout this Structure

1BHN is a Single protein structure of sequence from Bos taurus with as ligand. Active as Nucleoside-diphosphate kinase, with EC number 2.7.4.6 Known structural/functional Sites: , , , , and . Full crystallographic information is available from OCA.

ReferenceReference

The three-dimensional structures of two isoforms of nucleoside diphosphate kinase from bovine retina., Ladner JE, Abdulaev NG, Kakuev DL, Tordova M, Ridge KD, Gilliland GL, Acta Crystallogr D Biol Crystallogr. 1999 Jun;55(Pt 6):1127-35. PMID:10329774

Page seeded by OCA on Thu Feb 21 11:55:22 2008

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OCA

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 ==Overview==
-The crystal structures of two isoforms of nucleoside diphosphate kinase, from bovine retina overexpressed in Escherischia coli have been determined, to 2.4 A resolution. Both the isoforms, NBR-A and NBR-B, are hexameric and, the fold of the monomer is in agreement with NDP-kinase structures from, other biological sources. Although the polypeptide chains of the two, isoforms differ by only two residues, they crystallize in different space, groups. NBR-A crystallizes in space group P212121 with an entire hexamer, in the asymmetric unit, while NBR-B crystallizes in space group P43212, with a trimer in the asymmetric unit. The highly conserved, nucleotide-binding site observed in other nucleoside diphosphate kinase, structures is also observed here. Both NBR-A and NBR-B were crystallized, in the presence of cGMP. The nucleotide is bound with the base in the anti, conformation. The NBR-A active site contained both cGMP and GDP each bound, at half occupancy. Presumably, NBR-A had retained GDP (or GTP) from the, purification process. The NBR-B active site contained only cGMP.
+The crystal structures of two isoforms of nucleoside diphosphate kinase from bovine retina overexpressed in Escherischia coli have been determined to 2.4 A resolution. Both the isoforms, NBR-A and NBR-B, are hexameric and the fold of the monomer is in agreement with NDP-kinase structures from other biological sources. Although the polypeptide chains of the two isoforms differ by only two residues, they crystallize in different space groups. NBR-A crystallizes in space group P212121 with an entire hexamer in the asymmetric unit, while NBR-B crystallizes in space group P43212 with a trimer in the asymmetric unit. The highly conserved nucleotide-binding site observed in other nucleoside diphosphate kinase structures is also observed here. Both NBR-A and NBR-B were crystallized in the presence of cGMP. The nucleotide is bound with the base in the anti conformation. The NBR-A active site contained both cGMP and GDP each bound at half occupancy. Presumably, NBR-A had retained GDP (or GTP) from the purification process. The NBR-B active site contained only cGMP.
 ==About this Structure==
@@ Line 14: / Line 14: @@
 [[Category: Nucleoside-diphosphate kinase]]
 [[Category: Single protein]]
-[[Category: Abdulaev, N.G.]]
+[[Category: Abdulaev, N G.]]
 [[Category: Eisenstein, E.]]
-[[Category: Fujiwara, J.H.]]
+[[Category: Fujiwara, J H.]]
-[[Category: Gilliland, G.L.]]
+[[Category: Gilliland, G L.]]
-[[Category: Kakuev, D.L.]]
+[[Category: Kakuev, D L.]]
-[[Category: Karaschuk, G.N.]]
+[[Category: Karaschuk, G N.]]
-[[Category: Ladner, J.E.]]
+[[Category: Ladner, J E.]]
-[[Category: Ridge, K.D.]]
+[[Category: Ridge, K D.]]
 [[Category: Tordova, M.]]
 [[Category: 35G]]
 [[Category: phosphotransferase]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Feb  3 09:32:56 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 11:55:22 2008''