2gcv: Difference between revisions

Revision as of 18:30, 21 February 2008

Post-cleavage state of the Thermoanaerobacter tengcongensis glmS ribozyme

OverviewOverview

The glmS ribozyme is the only natural catalytic RNA known to require a small-molecule activator for catalysis. This catalytic RNA functions as a riboswitch, with activator-dependent RNA cleavage regulating glmS messenger RNA expression. We report crystal structures of the glmS ribozyme in precleavage states that are unliganded or bound to the competitive inhibitor glucose-6-phosphate and in the postcleavage state. All structures superimpose closely, revealing a remarkably rigid RNA that contains a preformed active and coenzyme-binding site. Unlike other riboswitches, the glmS ribozyme binds its activator in an open, solvent-accessible pocket. Our structures suggest that the amine group of the glmS ribozyme-bound coenzyme performs general acid-base and electrostatic catalysis.

About this StructureAbout this Structure

2GCV is a Single protein structure of sequence from [1] with and as ligands. Full crystallographic information is available from OCA.

ReferenceReference

Structural basis of glmS ribozyme activation by glucosamine-6-phosphate., Klein DJ, Ferre-D'Amare AR, Science. 2006 Sep 22;313(5794):1752-6. PMID:16990543

Page seeded by OCA on Thu Feb 21 17:30:38 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 4: / Line 4: @@
 ==Overview==
-The glmS ribozyme is the only natural catalytic RNA known to require a, small-molecule activator for catalysis. This catalytic RNA functions as a, riboswitch, with activator-dependent RNA cleavage regulating glmS, messenger RNA expression. We report crystal structures of the glmS, ribozyme in precleavage states that are unliganded or bound to the, competitive inhibitor glucose-6-phosphate and in the postcleavage state., All structures superimpose closely, revealing a remarkably rigid RNA that, contains a preformed active and coenzyme-binding site. Unlike other, riboswitches, the glmS ribozyme binds its activator in an open, solvent-accessible pocket. Our structures suggest that the amine group of, the glmS ribozyme-bound coenzyme performs general acid-base and, electrostatic catalysis.
+The glmS ribozyme is the only natural catalytic RNA known to require a small-molecule activator for catalysis. This catalytic RNA functions as a riboswitch, with activator-dependent RNA cleavage regulating glmS messenger RNA expression. We report crystal structures of the glmS ribozyme in precleavage states that are unliganded or bound to the competitive inhibitor glucose-6-phosphate and in the postcleavage state. All structures superimpose closely, revealing a remarkably rigid RNA that contains a preformed active and coenzyme-binding site. Unlike other riboswitches, the glmS ribozyme binds its activator in an open, solvent-accessible pocket. Our structures suggest that the amine group of the glmS ribozyme-bound coenzyme performs general acid-base and electrostatic catalysis.
 ==About this Structure==
@@ Line 12: / Line 12: @@
 Structural basis of glmS ribozyme activation by glucosamine-6-phosphate., Klein DJ, Ferre-D'Amare AR, Science. 2006 Sep 22;313(5794):1752-6. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16990543 16990543]
 [[Category: Single protein]]
-[[Category: Amare, A.R.Ferre-D.]]
+[[Category: Amare, A R.Ferre-D.]]
-[[Category: Klein, D.J.]]
+[[Category: Klein, D J.]]
 [[Category: MES]]
 [[Category: MG]]
 [[Category: rna; ribozyme; pseudoknot; helix]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jan 29 19:56:45 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:30:38 2008''