2cdg: Difference between revisions
New page: left|200px<br /><applet load="2cdg" size="350" color="white" frame="true" align="right" spinBox="true" caption="2cdg, resolution 2.60Å" /> '''STRUCTURE AND BINDIN... |
No edit summary |
||
| Line 4: | Line 4: | ||
==Overview== | ==Overview== | ||
Invariant human TCR Valpha24-Jalpha18+/Vbeta11+ NKT cells (iNKT) are | Invariant human TCR Valpha24-Jalpha18+/Vbeta11+ NKT cells (iNKT) are restricted by CD1d-alpha-glycosylceramides. We analyzed crystal structures and binding characteristics for an iNKT TCR plus two CD1d-alpha-GalCer-specific Vbeta11+ TCRs that use different TCR Valpha chains. The results were similar to those previously reported for MHC-peptide-specific TCRs, illustrating the versatility of the TCR platform. Docking TCR and CD1d-alpha-GalCer structures provided plausible insights into their interaction. The model supports a diagonal orientation of TCR on CD1d and suggests that complementarity determining region (CDR)3alpha, CDR3beta, and CDR1beta interact with ligands presented by CD1d, whereas CDR2beta binds to the CD1d alpha1 helix. This docking provides an explanation for the dominant usage of Vbeta11 and Vbeta8.2 chains by human and mouse iNKT cells, respectively, for recognition of CD1d-alpha-GalCer. | ||
==About this Structure== | ==About this Structure== | ||
| Line 14: | Line 14: | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Cerundolo, V.]] | [[Category: Cerundolo, V.]] | ||
[[Category: Gadola, S | [[Category: Gadola, S D.]] | ||
[[Category: Harlos, K.]] | [[Category: Harlos, K.]] | ||
[[Category: Jakobsen, B | [[Category: Jakobsen, B K.]] | ||
[[Category: Jones, E | [[Category: Jones, E Y.]] | ||
[[Category: Koch, M.]] | [[Category: Koch, M.]] | ||
[[Category: Lissin, N | [[Category: Lissin, N M.]] | ||
[[Category: Marles-Wright, J.]] | [[Category: Marles-Wright, J.]] | ||
[[Category: Matulis, G.]] | [[Category: Matulis, G.]] | ||
[[Category: Sheperd, D.]] | [[Category: Sheperd, D.]] | ||
[[Category: Stuart, D | [[Category: Stuart, D I.]] | ||
[[Category: Villiger, P | [[Category: Villiger, P M.]] | ||
[[Category: alpha-galactosylceramide]] | [[Category: alpha-galactosylceramide]] | ||
[[Category: cd1d]] | [[Category: cd1d]] | ||
| Line 35: | Line 35: | ||
[[Category: tcr 5b]] | [[Category: tcr 5b]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:47:31 2008'' | ||
Revision as of 17:47, 21 February 2008
|
STRUCTURE AND BINDING KINETICS OF THREE DIFFERENT HUMAN CD1D-ALPHA-GALACTOSYLCERAMIDE-SPECIFIC T CELL RECEPTORS (TCR 5B)
OverviewOverview
Invariant human TCR Valpha24-Jalpha18+/Vbeta11+ NKT cells (iNKT) are restricted by CD1d-alpha-glycosylceramides. We analyzed crystal structures and binding characteristics for an iNKT TCR plus two CD1d-alpha-GalCer-specific Vbeta11+ TCRs that use different TCR Valpha chains. The results were similar to those previously reported for MHC-peptide-specific TCRs, illustrating the versatility of the TCR platform. Docking TCR and CD1d-alpha-GalCer structures provided plausible insights into their interaction. The model supports a diagonal orientation of TCR on CD1d and suggests that complementarity determining region (CDR)3alpha, CDR3beta, and CDR1beta interact with ligands presented by CD1d, whereas CDR2beta binds to the CD1d alpha1 helix. This docking provides an explanation for the dominant usage of Vbeta11 and Vbeta8.2 chains by human and mouse iNKT cells, respectively, for recognition of CD1d-alpha-GalCer.
About this StructureAbout this Structure
2CDG is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
ReferenceReference
Structure and binding kinetics of three different human CD1d-alpha-galactosylceramide-specific T cell receptors., Gadola SD, Koch M, Marles-Wright J, Lissin NM, Shepherd D, Matulis G, Harlos K, Villiger PM, Stuart DI, Jakobsen BK, Cerundolo V, Jones EY, J Exp Med. 2006 Mar 20;203(3):699-710. Epub 2006 Mar 6. PMID:16520393
Page seeded by OCA on Thu Feb 21 16:47:31 2008
Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)
OCA- Pages with broken file links
- Homo sapiens
- Single protein
- Cerundolo, V.
- Gadola, S D.
- Harlos, K.
- Jakobsen, B K.
- Jones, E Y.
- Koch, M.
- Lissin, N M.
- Marles-Wright, J.
- Matulis, G.
- Sheperd, D.
- Stuart, D I.
- Villiger, P M.
- Alpha-galactosylceramide
- Cd1d
- Cell receptor
- Mhc class i
- Natural killer t cell
- Non-canonical
- Non-canonical natural killer tcr
- Tcr
- Tcr 5b