User:Nathan Harris/Tus: Difference between revisions
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=='''Structure of Tus protein and binding interactions with TerA'''== | =='''Structure of Tus protein and binding interactions with TerA'''== | ||
Tus constitutes 308 amino acid residues and a mass of approximately 36 kDa. The structural components of Tus have been elucidated through crystal structures of Tus bound to a fragment of ''TerA''. Tus exhibits a unique binding motif to Ter sites previously undescribed from any known protein-DNA interactions. | Tus constitutes 308 amino acid residues and a mass of approximately 36 kDa. The structural components of Tus have been elucidated through crystal structures of Tus bound to a fragment of ''TerA''. Tus exhibits a unique binding motif to Ter sites previously undescribed from any known protein-DNA interactions. | ||
Tus is divided into an amino and carboxy domain distinguished by two alpha helical regions and central β sheets combining to encompass a large central basic cleft. The interdomain region consists of anti-parallel β strands and an extended L4 loop which connect the amino and carboxy domains. Within this interdomain region, the βF, βG, βH and βI strands are responsible for specific and non-specific recognition of ''Ter''. | Tus is divided into an amino and carboxy domain distinguished by two alpha helical regions and central β sheets combining to encompass a large central basic cleft. The interdomain region consists of anti-parallel β strands and an extended L4 loop which connect the amino and carboxy domains. Within this interdomain region, the βF, βG, βH and βI strands are responsible for specific and non-specific recognition of ''Ter''. | ||