RTP and Tus: Difference between revisions
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DNA replication of circular bacterial chromosomes occurs using two replication forks that originate from one point (OriC), and move in opposite directions around the chromosome. In E. coli, B. Subtillis, and other bacteria and archaea, these replication forks are halted by interactions with terminator proteins bound to ''Ter'' sites. While it is possible for these organisms to not possess this type of replication-arrest mechanism, the conservation of this system across species indicates some sort of evolutionary benefit. | |||
This Replication Fork Arrest mechanism was first studied using the Tus protein from ''Escherichia coli'' and the Replication Termination Protein (RTP) from ''Bacillus subtillis''. Both of these proteins bind DNA sites known as "Terminator sites", or ''Ter'' sites. The termination of the replication fork is dependent on the direction of approach to these Ter sites: if the replication fork approaches from the permissive face replication will continue; however, if the replication fork approaches from the non-permissive face the fork will be arrested and DNA replication will cease at that point. | |||
== Tus: an asymmetric monomer, and unlikely candidate. == | |||