Sandbox Reserved 327: Difference between revisions
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=Structure= | =Structure= | ||
eIF1 is a small protein (12 kDa) that is encode by ''sui1'' which is one of the component of multifactor complex (MFC) that plays an important role in regulating translation initiation <ref name=" | eIF1 is a small protein (12 kDa) that is encode by ''sui1'' which is one of the component of multifactor complex (MFC) that plays an important role in regulating translation initiation <ref name="eIF1"/><ref name="asano"/>. eIF1 is a universal translation factor across organisms which make eIF1 homologs can be found in other eukayotes, archaea, and some bacteria <ref name="flet"/>. Yeast eIF1 contains a | ||
<scene name='Sandbox_Reserved_327/Ribbon_structure/1'>β-sheet with α helices</scene> | <scene name='Sandbox_Reserved_327/Ribbon_structure/1'>β-sheet with α helices</scene> | ||
on one side with N-terminal tail (aa 1-23) or NTT <ref name="eIF1"/>. It is homolog to | on one side with N-terminal tail (aa 1-23) or NTT <ref name="eIF1"/>. It is homolog to | ||
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==eIF1-ribosome binding site== | ==eIF1-ribosome binding site== | ||
The eIF1 would bind to the small subunit (40S) of a ribosome, in its P-site region. This binding would affect the conformation of the 40S subunit and the position of mRNA and the start codon initiation | The eIF1 would bind to the small subunit (40S) of a ribosome, in its P-site region. This binding would affect the conformation of the 40S subunit and the position of mRNA and the start codon initiation <ref name="lomakin"/>. Surprisingly, this binding site region is similar to the binding site of prokaryotic initiation factor IF3 to the ribosome, despite their unrelated structure <ref name="lomakin"/>. | ||
==eIF1-eIF5 binding sites== | ==eIF1-eIF5 binding sites== | ||
There are two | There are two | ||
<scene name='Sandbox_Reserved_327/Basic_eif1/3'>eIF1-eIF5 binding sites</scene>: at the NTT site of eIF1 and at a KH surface area, a specific region that rich in Lysine (K) and hydrophobic (H) residues <ref name="eIF1"/>. These bindings are different from the eIF1-ribosome binding site and are salt dependent, with high salt concentration would make the interaction weaker <ref name="eIF1"/>. The eIF1-NTT area is believed to play role in stimulating the MFC assembly by promoting the eIF5- eIF2β <ref name="eIF1"/>. The other binding site area (eIF1-KH) is believed to play role in the AUG start codon selection in translation initiation <ref name="eIF1"/>. | <scene name='Sandbox_Reserved_327/Basic_eif1/3'>eIF1-eIF5 binding sites</scene>: at the NTT site of eIF1 and at a KH surface area, a specific region that rich in Lysine (K) and hydrophobic (H) residues <ref name="eIF1"/>. These bindings are different from the eIF1-ribosome binding site and are salt dependent, with high salt concentration would make the interaction weaker <ref name="eIF1"/>. The eIF1-NTT area is believed to play role in stimulating the MFC assembly by promoting the eIF5- eIF2β <ref name="eIF1"/>. The other binding site area (eIF1-KH) is believed to play role in the AUG start codon selection in translation initiation <ref name="eIF1"/>. | ||
=Function and Mechanism= | =Function and Mechanism= | ||