Sandbox Reserved 327: Difference between revisions
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{{STRUCTURE_2ogh|PDB=2ogh|SCENE}} | {{STRUCTURE_2ogh|PDB=2ogh|SCENE}} | ||
=Structure | =Structure= | ||
eIF1 is a small protein (12 kDa) that is encode by ''sui1'' which is one of the component of multifactor complex (MFC) that plays an important role in regulating translation initiation <ref name="asano"/><ref name="eIF1"/>. eIF1 is a universal translation factor across organisms which make eIF1 homologs can be found in other eukayotes, archaea, and some bacteria <ref name="flet"/>. Yeast eIF1 contains a | eIF1 is a small protein (12 kDa) that is encode by ''sui1'' which is one of the component of multifactor complex (MFC) that plays an important role in regulating translation initiation <ref name="asano"/><ref name="eIF1"/>. eIF1 is a universal translation factor across organisms which make eIF1 homologs can be found in other eukayotes, archaea, and some bacteria <ref name="flet"/>. Yeast eIF1 contains a | ||
<scene name='Sandbox_Reserved_327/Ribbon_structure/1'>β-sheet with α helices</scene> | <scene name='Sandbox_Reserved_327/Ribbon_structure/1'>β-sheet with α helices</scene> | ||
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since the structure is similar to human eIF1 (87%) with 63% matched DNA composition (identity) <ref name="flet"/> <ref name="eIF1"/>. However, yeast eIF1 has two different conformations with two clear sets of backbone resonances for 13 of the 20 residues that lead to 20 different possible solution structure of yeast eIF1 <ref name="eIF1"/>. | since the structure is similar to human eIF1 (87%) with 63% matched DNA composition (identity) <ref name="flet"/> <ref name="eIF1"/>. However, yeast eIF1 has two different conformations with two clear sets of backbone resonances for 13 of the 20 residues that lead to 20 different possible solution structure of yeast eIF1 <ref name="eIF1"/>. | ||
=Function and Mechanism= | |||
[[Image:640px-Eukaryotic_initiation.png|thumb|left|Simplified eukaryotic initiation]] | |||
==AUG Start Codon Selection== | |||
== | |||
stimulates the recruitment of TC to the 40s subunit | stimulates the recruitment of TC to the 40s subunit | ||
*how it is released after AUG recognition? | |||
* | |||
==Multifactor Complex Assembly== | |||
=Binding sites and Mutations= | |||
==eIF1-ribosome== | |||
*binding to its P-site | |||
==eIF1-eIF5== | |||
<scene name='Sandbox_Reserved_327/Basic_eif1/3'>eIF5 binding site</scene> | <scene name='Sandbox_Reserved_327/Basic_eif1/3'>eIF5 binding site</scene> | ||
=Mutations= | ===Mutations=== | ||
==at the eIF1-eIF5 binding site | at the eIF1-eIF5 binding site | ||
*altering the basic part of eIF1-KH | *altering the basic part of eIF1-KH | ||
*altering the hydrophobic residues of eIF1 | *altering the hydrophobic residues of eIF1 | ||
=References= | =References= | ||