Prp24: Difference between revisions
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Prp24 is a U6 snNRP protein that functions in the annealing of the U4 and U6 snRNPs during the assembly of the spliceosome. This protein was first identified in a genetic screen as a mutated gene that caused an accumulation of pre-mRNA <ref name="Vij">PMID:2676722</ref>. Its first functional role was suggested after several mutant forms of the protein were found to suppress a cold sensitive growth defect caused by mutations in the U4 snRNA <ref name="Shannon">PMID:1827420</ref>. It is thought that Prp24 helps to stabilize the U6 snRNA and hold it in a conformation that promotes base pairing interactions with the U4 snRNA to form the stem I and stem II structures of the U4/U6 di-snRNP <ref name="Vidaver">PMID:10545453</ref>. Although Prp24 departs from the U4/U6 complex before the formation of the U4/U6.U5 tri-snRNP, it has been suggested that Prp24 may also play a role in the dissociation of U4 from U6 during the base pairing of U6 with U2 and the 5' splice site <ref name="Vaidya">Vaidya V, Vijayraghavan U. Prp21, a U2-snRNP-associated protein, and Prp24, a U6-snRNP-associated protein, functionally interact during spliceosome assembly. J Genet. 1998 Dec; 77(3):85-94</ref>. This additional role for Prp24, however, has not been sufficiently supported experimentally. | Prp24 is a U6 snNRP protein that functions in the annealing of the U4 and U6 snRNPs during the assembly of the spliceosome. This protein was first identified in a genetic screen as a mutated gene that caused an accumulation of pre-mRNA <ref name="Vij">PMID:2676722</ref>. Its first functional role was suggested after several mutant forms of the protein were found to suppress a cold sensitive growth defect caused by mutations in the U4 snRNA <ref name="Shannon">PMID:1827420</ref>. It is thought that Prp24 helps to stabilize the U6 snRNA and hold it in a conformation that promotes base pairing interactions with the U4 snRNA to form the stem I and stem II structures of the U4/U6 di-snRNP <ref name="Vidaver">PMID:10545453</ref>. Although Prp24 departs from the U4/U6 complex before the formation of the U4/U6.U5 tri-snRNP, it has been suggested that Prp24 may also play a role in the dissociation of U4 from U6 during the base pairing of U6 with U2 and the 5' splice site <ref name="Vaidya">Vaidya V, Vijayraghavan U. Prp21, a U2-snRNP-associated protein, and Prp24, a U6-snRNP-associated protein, functionally interact during spliceosome assembly. J Genet. 1998 Dec; 77(3):85-94</ref>. This additional role for Prp24, however, has not been sufficiently supported experimentally. | ||
== Structure == | == Structure == | ||