Prp24: Difference between revisions
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===RNA Recognition Motifs=== | ===RNA Recognition Motifs=== | ||
Prp24 contains four RRMs, RRM 1, RRM 2, RRM 3, and RRM 4. These motifs have a <scene name='Sandbox_Reserved_340/2ghp/ | Prp24 contains four RRMs, RRM 1, RRM 2, RRM 3, and RRM 4. These motifs have a<scene name='Sandbox_Reserved_340/2ghp/7'>canonical structure of a platform of four β-strands with two α-helices on one side of the β-sheet plane</scene>. These RRMs are present in many proteins that bind to to single stranded regions of RNA <ref name="RRM"/> and their presence in Prp24 supports a role for the annealing of U4 and U6 snRNAs into the U4/U6 structure. | ||
Within each RRM, there are RNP consensus domains <ref name="RRM">PMID:15853797</ref>. These are the regions in the β-strands that are thought to actually interact with the RNA <ref name="RRM"/>. These regions seem to be very important in Prp24 and its interaction with U4 and U6. The study that first identified a probable link between the Prp24 protein and U4/U6 found that mutations in RNP 1 and RNP 2 of the carboxy terminal RRM (green link) rescued a cold-sensitive phenotype caused by a U4 mutation in stem II of U4/U6 <ref name="Shannon"/>. Two further studies <ref name="Vidaver"/> <ref name="Kwan">PMID:15811912</ref> showed that the mutation of three highly conserved residues in the RNP domains of any of the four RRMs (green link) conferred either temperature-sensitive growth or lethality to yeast cells. | Within each RRM, there are RNP consensus domains <ref name="RRM">PMID:15853797</ref>. These are the regions in the β-strands that are thought to actually interact with the RNA <ref name="RRM"/>. These regions seem to be very important in Prp24 and its interaction with U4 and U6. The study that first identified a probable link between the Prp24 protein and U4/U6 found that mutations in RNP 1 and RNP 2 of the carboxy terminal RRM (green link) rescued a cold-sensitive phenotype caused by a U4 mutation in stem II of U4/U6 <ref name="Shannon"/>. Two further studies <ref name="Vidaver"/> <ref name="Kwan">PMID:15811912</ref> showed that the mutation of three highly conserved residues in the RNP domains of any of the four RRMs (green link) conferred either temperature-sensitive growth or lethality to yeast cells. | ||