2e2s: Difference between revisions
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==Overview== | ==Overview== | ||
Agelenin, isolated from the Agelenidae spider Agelena opulenta, is a | Agelenin, isolated from the Agelenidae spider Agelena opulenta, is a peptide composed of 35 amino acids. We determined the three-dimensional structure of agelenin using two-dimensional NMR spectroscopy. The structure is composed of a short antiparallel beta-sheet and four beta-turns, which are stabilized by three disulfide bonds. Agelenin has characteristic residues, Phe9, Ser28 and Arg33, which are arranged similarly to the pharmacophore of the insect channel inhibitor, omega-atracotoxin-Hv1a. These observations suggest that agelenin and omega-atracotoxin-Hv1a bind to insect calcium channels in a similar manner. We also suggest that another mode of action may operate in the channel inhibition by omega-agatoxin-IVA and omega-atracotoxin-Hv2a. | ||
==About this Structure== | ==About this Structure== | ||
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[[Category: toxin]] | [[Category: toxin]] | ||
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Revision as of 18:05, 21 February 2008
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Solution structure of agelenin, an insecticidal peptide from the venom of Agelena opulenta
OverviewOverview
Agelenin, isolated from the Agelenidae spider Agelena opulenta, is a peptide composed of 35 amino acids. We determined the three-dimensional structure of agelenin using two-dimensional NMR spectroscopy. The structure is composed of a short antiparallel beta-sheet and four beta-turns, which are stabilized by three disulfide bonds. Agelenin has characteristic residues, Phe9, Ser28 and Arg33, which are arranged similarly to the pharmacophore of the insect channel inhibitor, omega-atracotoxin-Hv1a. These observations suggest that agelenin and omega-atracotoxin-Hv1a bind to insect calcium channels in a similar manner. We also suggest that another mode of action may operate in the channel inhibition by omega-agatoxin-IVA and omega-atracotoxin-Hv2a.
About this StructureAbout this Structure
2E2S is a Single protein structure of sequence from Agelena opulenta. Full crystallographic information is available from OCA.
ReferenceReference
Solution structure of agelenin, an insecticidal peptide isolated from the spider Agelena opulenta, and its structural similarities to insect-specific calcium channel inhibitors., Yamaji N, Sugase K, Nakajima T, Miki T, Wakamori M, Mori Y, Iwashita T, FEBS Lett. 2007 Aug 7;581(20):3789-94. Epub 2007 Jul 10. PMID:17644092
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