Colicin E7: Difference between revisions
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Colicin E7 is a type of [[Colicin]], a bacteriocin made by ''E. coli'' which acts against other nearby ''E. coli'' to kill them with its DNase activity; it digests the cell's genome in specific locations, ultimately leading to the death of the cell. | Colicin E7 is a type of [[Colicin]], a bacteriocin made by ''E. coli'' which acts against other nearby ''E. coli'' to kill them with its [[DNase activity]]; it digests the cell's genome in specific locations, ultimately leading to the death of the cell. | ||
==Synthesis and release== | ==Synthesis and release== | ||
After synthesis inside the ''E. coli'' cell, the colicin binds its | After synthesis inside the ''E. coli'' cell, the colicin binds its [[Colicin Immunity Protein]], [[Im7]], to its nuclease domain, to prevent the host cell from being killed by its activity. This immunity protein is released only on binding to the outer membrane of the target cell. | ||
==Mechanism of uptake== | ==Mechanism of uptake== | ||
{{STRUCTURE_2axc | PDB=2axc | SCENE= Colicin_E7/Cole7t/1 }} | {{STRUCTURE_2axc | PDB=2axc | SCENE= Colicin_E7/Cole7t/1 }} | ||
This structure shows the translocation domain of ColE7. | |||
The receptor binding domain of ColE7 binds to the [[BtuB]] vitamin B12 receptor on the outer membrane of the target cell. This causes the dissociation of the immunity protein from the cytotoxic domain of the colicin, allowing it to target the cell when it enters. Binding BtuB triggers the recruitment of [[OmpF]] and the TolQRAB complex, to bind to the translocation domain and translocate the colicin across the membrane. The mechanism by which this occurs has not yet been identified. This structure shows the translocation domain of ColE7. | |||
==Killing Activities== | ==Killing Activities== | ||