Colicin Ia: Difference between revisions
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Insertion of ColIa into the lipid membrane triggers a series of conformational change across the whole of ColIa to form the ion channel<ref> PMID: 10845238 </ref>, probably through a series of intermediates. While a monomer of ColIa in the membrane is sufficient to form the channel and kill the cell, studies have shown that the channel, when enough molecules are present, exists as a multimer with a larger pore diameter<ref> PMID: 9729746 </ref><ref> PMID: 19357078 </ref>. | Insertion of ColIa into the lipid membrane triggers a series of conformational change across the whole of ColIa to form the ion channel<ref> PMID: 10845238 </ref>, probably through a series of intermediates. While a monomer of ColIa in the membrane is sufficient to form the channel and kill the cell, studies have shown that the channel, when enough molecules are present, exists as a multimer with a larger pore diameter<ref> PMID: 9729746 </ref><ref> PMID: 19357078 </ref>. | ||
Once the pore has inserted into the membrane and is functioning fully, it inhibits the active transport of a number of molecules, primarily proline, thiomethyl-β-D-galactoside, and K<sup>+</sup> ions. Not only does it prevent their uptake, it can also trigger the rapid release of the molecules. It also inhibits succinate uptake, which accounts for the inhibition of respiration observed in targeted cells, and has an effect on the uptake of glycerol<ref> PMID: 1091634 </ref>. | Once the pore has inserted into the membrane and is functioning fully, it inhibits the active transport of a number of molecules, primarily proline, thiomethyl-β-D-galactoside, and K<sup>+</sup> ions. Not only does it prevent their uptake, it can also trigger the rapid release of the molecules. It also inhibits succinate uptake, which accounts for the inhibition of respiration observed in targeted cells, and has an effect on the uptake of glycerol<ref> PMID: 1091634 </ref>. All of this leads to an uncoupling of electron transport from active transport, and affects energy metabolism with its interference with the membrane, leading to a depolarisation of the membrane<ref> PMID: 388932 </ref><ref> PMID: 26912 </ref><ref> PMID: 779829 </ref>. | ||
==References== | ==References== | ||
<references/> | <references/> | ||