Colicin E9: Difference between revisions

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 The DNase domain nicks dsDNA at thymine bases<ref> PMID: 15995205 </ref>.
-The catalytic centre of the DNase domain contains the [[H-N-H motif]], a site for DNA and metal (zinc or nickel ion) binding. Binding zinc stabilises the protein<ref> PMID: 15044477 </ref>, and regulates the binding of phosphate ions to the active site. Upon binding to the metal ion, the conformation of the DNase alters. This diagram shows the binding of the metal ion and the phosphate to the DNase domain<ref> PMID: 15726170 </ref>.
+The catalytic centre of the DNase domain contains the [[H-N-H motif]], a site for DNA and metal binding. ColE9 binds Mg<super>2+</super> as its cofactor<ref> PMID: 12136104 </ref>. Binding the magnesium ion stabilises the protein<ref> PMID: 15044477 </ref>, and regulates the binding of phosphate ions to the active site. Upon binding to the ion, the conformation of the DNase alters. This diagram shows the binding of the metal ion and the phosphate to the DNase domain<ref> PMID: 15726170 </ref>.
 [[Image:From_15726170.png]]