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{{STRUCTURE_3k3q| PDB=3k3q | SCENE= }} | {{STRUCTURE_3k3q| PDB=3k3q | SCENE= }} | ||
===Crystal Structure of a Llama Antibody complexed with the C. Botulinum Neurotoxin Serotype A Catalytic Domain=== | ===Crystal Structure of a Llama Antibody complexed with the C. Botulinum Neurotoxin Serotype A Catalytic Domain=== | ||
{{ABSTRACT_PUBMED_20138889}} | |||
==Function== | |||
[[http://www.uniprot.org/uniprot/BXA1_CLOBH BXA1_CLOBH]] Inhibits acetylcholine release. The botulinum toxin binds with high affinity to peripheral neuronal presynaptic membrane to the secretory vesicle protein SV2. It binds directly to the largest luminal loop of SV2A, SV2B and SV2C. It is then internalized by receptor-mediated endocytosis. The C-terminus of the heavy chain (H) is responsible for the adherence of the toxin to the cell surface while the N-terminus mediates transport of the light chain from the endocytic vesicle to the cytosol. After translocation, the light chain (L) hydrolyzes the 197-Gln-|-Arg-198 bond in SNAP-25, thereby blocking neurotransmitter release. Inhibition of acetylcholine release results in flaccid paralysis, with frequent heart or respiratory failure. | |||
-- | |||
==About this Structure== | ==About this Structure== | ||
[[3k3q]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Clostridium_botulinum_a_str._hall Clostridium botulinum a str. hall] and [http://en.wikipedia.org/wiki/Lama_glama Lama glama]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3K3Q OCA]. | [[3k3q]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Clostridium_botulinum_a_str._hall Clostridium botulinum a str. hall] and [http://en.wikipedia.org/wiki/Lama_glama Lama glama]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3K3Q OCA]. | ||
==Reference== | ==Reference== | ||
<ref group="xtra">PMID: | <ref group="xtra">PMID:020138889</ref><references group="xtra"/><references/> | ||
[[Category: Bontoxilysin]] | [[Category: Bontoxilysin]] | ||
[[Category: Clostridium botulinum a str. hall]] | [[Category: Clostridium botulinum a str. hall]] | ||
| Line 38: | Line 23: | ||
[[Category: Cell junction]] | [[Category: Cell junction]] | ||
[[Category: Cell membrane]] | [[Category: Cell membrane]] | ||
[[Category: Disulfide bond]] | [[Category: Disulfide bond]] | ||
[[Category: Hydrolase]] | [[Category: Hydrolase]] | ||
| Line 47: | Line 31: | ||
[[Category: Metalloprotease]] | [[Category: Metalloprotease]] | ||
[[Category: Neurotoxin]] | [[Category: Neurotoxin]] | ||
[[Category: Protease]] | [[Category: Protease]] | ||
[[Category: Secreted]] | [[Category: Secreted]] | ||
| Line 54: | Line 37: | ||
[[Category: Transmembrane]] | [[Category: Transmembrane]] | ||
[[Category: Vhh]] | [[Category: Vhh]] | ||
Revision as of 05:31, 4 April 2013
Crystal Structure of a Llama Antibody complexed with the C. Botulinum Neurotoxin Serotype A Catalytic DomainCrystal Structure of a Llama Antibody complexed with the C. Botulinum Neurotoxin Serotype A Catalytic Domain
Template:ABSTRACT PUBMED 20138889
FunctionFunction
[BXA1_CLOBH] Inhibits acetylcholine release. The botulinum toxin binds with high affinity to peripheral neuronal presynaptic membrane to the secretory vesicle protein SV2. It binds directly to the largest luminal loop of SV2A, SV2B and SV2C. It is then internalized by receptor-mediated endocytosis. The C-terminus of the heavy chain (H) is responsible for the adherence of the toxin to the cell surface while the N-terminus mediates transport of the light chain from the endocytic vesicle to the cytosol. After translocation, the light chain (L) hydrolyzes the 197-Gln-|-Arg-198 bond in SNAP-25, thereby blocking neurotransmitter release. Inhibition of acetylcholine release results in flaccid paralysis, with frequent heart or respiratory failure.
About this StructureAbout this Structure
3k3q is a 3 chain structure with sequence from Clostridium botulinum a str. hall and Lama glama. Full crystallographic information is available from OCA.
ReferenceReference
- ↑ Dong J, Thompson AA, Fan Y, Lou J, Conrad F, Ho M, Pires-Alves M, Wilson BA, Stevens RC, Marks JD. A single-domain llama antibody potently inhibits the enzymatic activity of botulinum neurotoxin by binding to the non-catalytic alpha-exosite binding region. J Mol Biol. 2010 Apr 9;397(4):1106-18. Epub 2010 Feb 6. PMID:20138889 doi:10.1016/j.jmb.2010.01.070