2q2t: Difference between revisions
New page: left|200px<br /><applet load="2q2t" size="350" color="white" frame="true" align="right" spinBox="true" caption="2q2t, resolution 2.300Å" /> '''Structure of Chlore... |
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==Overview== | ==Overview== | ||
Chlorella virus DNA ligase, the smallest eukaryotic ligase known, has | Chlorella virus DNA ligase, the smallest eukaryotic ligase known, has pluripotent biological activity and an intrinsic nick-sensing function, despite having none of the accessory domains found in cellular ligases. A 2.3-A crystal structure of the Chlorella virus ligase-AMP intermediate bound to duplex DNA containing a 3'-OH-5'-PO4 nick reveals a new mode of DNA envelopment, in which a short surface loop emanating from the OB domain forms a beta-hairpin 'latch' that inserts into the DNA major groove flanking the nick. A network of interactions with the 3'-OH and 5'-PO4 termini in the active site illuminates the DNA adenylylation mechanism and the crucial roles of AMP in nick sensing and catalysis. Addition of a divalent cation triggered nick sealing in crystallo, establishing that the nick complex is a bona fide intermediate in the DNA repair pathway. | ||
==About this Structure== | ==About this Structure== | ||
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[[Category: Paramecium bursaria chlorella virus 1]] | [[Category: Paramecium bursaria chlorella virus 1]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Lima, C | [[Category: Lima, C D.]] | ||
[[Category: Nair, P | [[Category: Nair, P A.]] | ||
[[Category: Nandakumar, J.]] | [[Category: Nandakumar, J.]] | ||
[[Category: Shuman, S.]] | [[Category: Shuman, S.]] | ||
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[[Category: protein-dna complex]] | [[Category: protein-dna complex]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:35:19 2008'' | ||
