1uzr: Difference between revisions
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Revision as of 17:12, 30 October 2007
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CRYSTAL STRUCTURE OF THE CLASS IB RIBONUCLEOTIDE REDUCTASE R2F-2 SUBUNIT FROM MYCOBACTERIUM TUBERCULOSIS
OverviewOverview
Two nrdF genes of Mycobacterium tuberculosis code for different R2, subunits of the class Ib ribonucleotide reductase (RNR). The proteins are, denoted R2F-1 and R2F-2 having 71% sequence identity. The R2F-2 subunit, forms the biologically active RNR complex with the catalytic R1E-subunit., We present the structure of the reduced R2F-2 subunit to 2.2 A resolution., Comparison of the R2F-2 structure with a model of R2F-1 suggests that the, important differences are located at the C-terminus. We found that within, class Ib, the E-helix close to the iron diiron centre has two preferred, conformations, which cannot be explained by the redox-state of the diiron, centre. In the R2F-2 structure, we also could see a mobility of alphaE in, between the two conformations.
About this StructureAbout this Structure
1UZR is a [Single protein] structure of sequence from [Mycobacterium tuberculosis] with FE, CIT and GOL as [ligands]. Active as [Ribonucleoside-diphosphate reductase], with EC number [1.17.4.1]. Structure known Active Site: AC1. Full crystallographic information is available from [OCA].
ReferenceReference
Crystal structure of the biologically active form of class Ib ribonucleotide reductase small subunit from Mycobacterium tuberculosis., Uppsten M, Davis J, Rubin H, Uhlin U, FEBS Lett. 2004 Jul 2;569(1-3):117-22. PMID:15225619
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