2r6a: Difference between revisions
New page: left|200px<br /><applet load="2r6a" size="350" color="white" frame="true" align="right" spinBox="true" caption="2r6a, resolution 2.90Å" /> '''Crystal Form BH1'''<... |
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==Overview== | ==Overview== | ||
The complex between the DnaB helicase and the DnaG primase unwinds duplex | The complex between the DnaB helicase and the DnaG primase unwinds duplex DNA at the eubacterial replication fork and synthesizes the Okazaki RNA primers. The crystal structures of hexameric DnaB and its complex with the helicase binding domain (HBD) of DnaG reveal that within the hexamer the two domains of DnaB pack with strikingly different symmetries to form a distinct two-layered ring structure. Each of three bound HBDs stabilizes the DnaB hexamer in a conformation that may increase its processivity. Three positive, conserved electrostatic patches on the N-terminal domain of DnaB may also serve as a binding site for DNA and thereby guide the DNA to a DnaG active site. | ||
==About this Structure== | ==About this Structure== | ||
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[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Bailey, S.]] | [[Category: Bailey, S.]] | ||
[[Category: Eliason, W | [[Category: Eliason, W K.]] | ||
[[Category: Steitz, T | [[Category: Steitz, T A.]] | ||
[[Category: SO4]] | [[Category: SO4]] | ||
[[Category: dnab]] | [[Category: dnab]] | ||
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[[Category: replication]] | [[Category: replication]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:44:55 2008'' | ||
Revision as of 19:44, 21 February 2008
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Crystal Form BH1
OverviewOverview
The complex between the DnaB helicase and the DnaG primase unwinds duplex DNA at the eubacterial replication fork and synthesizes the Okazaki RNA primers. The crystal structures of hexameric DnaB and its complex with the helicase binding domain (HBD) of DnaG reveal that within the hexamer the two domains of DnaB pack with strikingly different symmetries to form a distinct two-layered ring structure. Each of three bound HBDs stabilizes the DnaB hexamer in a conformation that may increase its processivity. Three positive, conserved electrostatic patches on the N-terminal domain of DnaB may also serve as a binding site for DNA and thereby guide the DNA to a DnaG active site.
About this StructureAbout this Structure
2R6A is a Protein complex structure of sequences from Geobacillus stearothermophilus with as ligand. Full crystallographic information is available from OCA.
ReferenceReference
Structure of hexameric DnaB helicase and its complex with a domain of DnaG primase., Bailey S, Eliason WK, Steitz TA, Science. 2007 Oct 19;318(5849):459-63. PMID:17947583
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