AZT-resistant HIV-1 reverse transcriptase: Difference between revisions
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[[Reverse transcriptase]] is a heterodimer of two related <scene name='AZT-resistant_HIV-1_reverse_transcriptase/Subunits/1'>subunits</scene>: p66 and p51. The p66 subunit has 560 amino acid residues, while the p51 subunit has 440 amino acids. The larger subunit, p66, contains the active sites for both enzymatic activities (DNA polymerase and RNase H), while the smaller subunit, p51, has a structural role. | [[Reverse transcriptase]] is a heterodimer of two related <scene name='AZT-resistant_HIV-1_reverse_transcriptase/Subunits/1'>subunits</scene>: p66 and p51. The p66 subunit has 560 amino acid residues, while the p51 subunit has 440 amino acids. The larger subunit, p66, contains the active sites for both enzymatic activities (DNA polymerase and RNase H), while the smaller subunit, p51, has a structural role. | ||
Two distinct domains, polymerase and RNase H form the p66 subunit. The polymerase domain consists of four subdomains: | Two distinct domains, polymerase and RNase H form the p66 subunit. The polymerase domain consists of four subdomains: fingers (residues 1-85 and 118-155), palm (residues 86-117 and 156-236), thumb (residues 237-318), and connection (residues 319-426). The p51 subunit folds into the same subdomains, but the positions of the subdomains relative to each other are different. | ||
[[Image:RT finger.png]] | [[Image:RT finger.png]] | ||
''' | '''Fingers Subdomain''' | ||
The nucleic-acid binding cleft is primarily formed by the p66 [[polymerase]] and RNase H subdomains. The p51 thumb and connection subdomains form the floor of the binding cleft. In this way, the binding cleft is positioned so that both the polymerase and the RNAse H active sites come in contact with the nucleic acid. | |||
==Inhibition of Reverse Transcriptase Activity== | ==Inhibition of Reverse Transcriptase Activity== | ||