2ze9: Difference between revisions

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Crystal structure of H168A mutant of phospholipase D from Streptomyces antibioticus, as a complex with phosphatidylcholine

OverviewOverview

Phospholipase D (E.C. 3.1.4.4) from Streptomyces antibioticus has been crystallized in six crystal forms using the hanging-drop vapour-diffusion method. The type III and V crystals belong to monoclinic and hexagonal systems, respectively. All of the other crystal forms, types I, II, IV and VI, belong to orthorhombic space group P212121. Of these four types, the type VI crystals are suitable for X-ray structure determination. Crystal data for type VI crystals are: a = 50.1, b = 98.7, c = 107.6 A, V = 532100 A3, Z = 4 and Vm = 2.47 A3 Da-1. Type VI crystals diffract to at least 2.3 A resolution. A total of 11295 independent reflections to 3 A resolution have been collected from a type VI crystal using a conventional X-ray source, and its structural analysis is currently being conducted using isomorphous replacement methods.

About this StructureAbout this Structure

2ZE9 is a Single protein structure of sequence from Streptomyces antibioticus with and as ligands. Active as Phospholipase D, with EC number 3.1.4.4 Full crystallographic information is available from OCA.

ReferenceReference

Crystallization and preliminary X-ray diffraction studies of phospholipase D from Streptomyces antibioticus., Suzuki A, Kakuno K, Iwasaki Y, Yamane T, Yamane T, Acta Crystallogr D Biol Crystallogr. 1999 Jan;55(Pt 1):317-9. Epub 1999, Jan 1. PMID:10089437

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 ==Overview==
-Phospholipase D (E.C. 3.1.4.4) from Streptomyces antibioticus has been, crystallized in six crystal forms using the hanging-drop vapour-diffusion, method. The type III and V crystals belong to monoclinic and hexagonal, systems, respectively. All of the other crystal forms, types I, II, IV and, VI, belong to orthorhombic space group P212121. Of these four types, the, type VI crystals are suitable for X-ray structure determination. Crystal, data for type VI crystals are: a = 50.1, b = 98.7, c = 107.6 A, V = 532100, A3, Z = 4 and Vm = 2.47 A3 Da-1. Type VI crystals diffract to at least 2.3, A resolution. A total of 11295 independent reflections to 3 A resolution, have been collected from a type VI crystal using a conventional X-ray, source, and its structural analysis is currently being conducted using, isomorphous replacement methods.
+Phospholipase D (E.C. 3.1.4.4) from Streptomyces antibioticus has been crystallized in six crystal forms using the hanging-drop vapour-diffusion method. The type III and V crystals belong to monoclinic and hexagonal systems, respectively. All of the other crystal forms, types I, II, IV and VI, belong to orthorhombic space group P212121. Of these four types, the type VI crystals are suitable for X-ray structure determination. Crystal data for type VI crystals are: a = 50.1, b = 98.7, c = 107.6 A, V = 532100 A3, Z = 4 and Vm = 2.47 A3 Da-1. Type VI crystals diffract to at least 2.3 A resolution. A total of 11295 independent reflections to 3 A resolution have been collected from a type VI crystal using a conventional X-ray source, and its structural analysis is currently being conducted using isomorphous replacement methods.
 ==About this Structure==
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 [[Category: secreted]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 23 11:29:26 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 19:01:42 2008''