2p3m: Difference between revisions
New page: left|200px<br /><applet load="2p3m" size="350" color="white" frame="true" align="right" spinBox="true" caption="2p3m" /> '''Solution structure of Mj0056'''<br /> ==Ove... |
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==Overview== | ==Overview== | ||
Proteins of the cradle-loop barrel metafold are formed by duplication of a | Proteins of the cradle-loop barrel metafold are formed by duplication of a conserved betaalphabeta-element, suggesting a common evolutionary origin from an ancestral group of nucleic acid-binding proteins. The basal fold within this metafold, the RIFT barrel, is also found in a wide range of enzymes, whose homologous relationship with the nucleic acid-binding group is unclear. We have characterized a protein family that is intermediate in sequence and structure between the basal group of cradle-loop barrels and one family of RIFT-barrel enzymes, the riboflavin kinases. We report the structure, substrate-binding mode, and catalytic activity for one of these proteins, Methanocaldococcus jannaschii Mj0056, which is an archaeal riboflavin kinase. Mj0056 is unusual in utilizing CTP rather than ATP as the donor nucleotide, and sequence conservation in the relevant residues suggests that this is a general feature of archaeal riboflavin kinases. | ||
==About this Structure== | ==About this Structure== | ||
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==Reference== | ==Reference== | ||
A CTP- | A CTP-dependent archaeal riboflavin kinase forms a bridge in the evolution of cradle-loop barrels., Ammelburg M, Hartmann MD, Djuranovic S, Alva V, Koretke KK, Martin J, Sauer G, Truffault V, Zeth K, Lupas AN, Coles M, Structure. 2007 Dec;15(12):1577-90. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=18073108 18073108] | ||
[[Category: Methanocaldococcus jannaschii]] | [[Category: Methanocaldococcus jannaschii]] | ||
[[Category: Riboflavin kinase]] | [[Category: Riboflavin kinase]] | ||
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[[Category: Coles, M.]] | [[Category: Coles, M.]] | ||
[[Category: Djuranovic, S.]] | [[Category: Djuranovic, S.]] | ||
[[Category: Lupas, A | [[Category: Lupas, A N.]] | ||
[[Category: Martin, J.]] | [[Category: Martin, J.]] | ||
[[Category: Truffault, V.]] | [[Category: Truffault, V.]] | ||
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[[Category: rift barrel]] | [[Category: rift barrel]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:25:33 2008'' | ||
Revision as of 19:25, 21 February 2008
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Solution structure of Mj0056
OverviewOverview
Proteins of the cradle-loop barrel metafold are formed by duplication of a conserved betaalphabeta-element, suggesting a common evolutionary origin from an ancestral group of nucleic acid-binding proteins. The basal fold within this metafold, the RIFT barrel, is also found in a wide range of enzymes, whose homologous relationship with the nucleic acid-binding group is unclear. We have characterized a protein family that is intermediate in sequence and structure between the basal group of cradle-loop barrels and one family of RIFT-barrel enzymes, the riboflavin kinases. We report the structure, substrate-binding mode, and catalytic activity for one of these proteins, Methanocaldococcus jannaschii Mj0056, which is an archaeal riboflavin kinase. Mj0056 is unusual in utilizing CTP rather than ATP as the donor nucleotide, and sequence conservation in the relevant residues suggests that this is a general feature of archaeal riboflavin kinases.
About this StructureAbout this Structure
2P3M is a Single protein structure of sequence from Methanocaldococcus jannaschii. Active as Riboflavin kinase, with EC number 2.7.1.26 Full crystallographic information is available from OCA.
ReferenceReference
A CTP-dependent archaeal riboflavin kinase forms a bridge in the evolution of cradle-loop barrels., Ammelburg M, Hartmann MD, Djuranovic S, Alva V, Koretke KK, Martin J, Sauer G, Truffault V, Zeth K, Lupas AN, Coles M, Structure. 2007 Dec;15(12):1577-90. PMID:18073108
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