3bue: Difference between revisions

Revision as of 20:07, 21 February 2008

Crystal structure of the C-terminal domain hexamer of ArgR from Mycobacterium tuberculosis

OverviewOverview

The gene product of an open reading frame Rv1657 from Mycobacterium tuberculosis is a putative arginine repressor protein (ArgR), a transcriptional factor that regulates the expression of arginine-biosynthetic enzymes. Rv1657 was expressed and purified and a C-terminal domain was crystallized using the hanging-drop vapour-diffusion method. Diffraction data were collected and processed to a resolution of 2.15 A. The crystals belong to space group P1 and the Matthews coefficient suggests that the crystals contain six C-terminal domain molecules per unit cell. Previous structural and biochemical studies on the arginine repressor proteins from other organisms have likewise shown the presence of six molecules per unit cell.

About this StructureAbout this Structure

3BUE is a Single protein structure of sequence from Mycobacterium tuberculosis h37rv. Full crystallographic information is available from OCA.

ReferenceReference

Expression, purification and preliminary X-ray analysis of the C-terminal domain of an arginine repressor protein from Mycobacterium tuberculosis., Lu GJ, Garen CR, Cherney MM, Cherney LT, Lee C, James MN, Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 Nov 1;63(Pt, 11):936-9. Epub 2007 Oct 24. PMID:18007044

Page seeded by OCA on Thu Feb 21 19:07:38 2008

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OCA

@@ Line 4: / Line 4: @@
 ==Overview==
-The gene product of an open reading frame Rv1657 from Mycobacterium, tuberculosis is a putative arginine repressor protein (ArgR), a, transcriptional factor that regulates the expression of, arginine-biosynthetic enzymes. Rv1657 was expressed and purified and a, C-terminal domain was crystallized using the hanging-drop vapour-diffusion, method. Diffraction data were collected and processed to a resolution of, 2.15 A. The crystals belong to space group P1 and the Matthews coefficient, suggests that the crystals contain six C-terminal domain molecules per, unit cell. Previous structural and biochemical studies on the arginine, repressor proteins from other organisms have likewise shown the presence, of six molecules per unit cell.
+The gene product of an open reading frame Rv1657 from Mycobacterium tuberculosis is a putative arginine repressor protein (ArgR), a transcriptional factor that regulates the expression of arginine-biosynthetic enzymes. Rv1657 was expressed and purified and a C-terminal domain was crystallized using the hanging-drop vapour-diffusion method. Diffraction data were collected and processed to a resolution of 2.15 A. The crystals belong to space group P1 and the Matthews coefficient suggests that the crystals contain six C-terminal domain molecules per unit cell. Previous structural and biochemical studies on the arginine repressor proteins from other organisms have likewise shown the presence of six molecules per unit cell.
 ==About this Structure==
@@ Line 13: / Line 13: @@
 [[Category: Mycobacterium tuberculosis h37rv]]
 [[Category: Single protein]]
-[[Category: Cherney, L.T.]]
+[[Category: Cherney, L T.]]
-[[Category: Cherney, M.M.]]
+[[Category: Cherney, M M.]]
-[[Category: Garen, C.R.]]
+[[Category: Garen, C R.]]
-[[Category: James, M.N.G.]]
+[[Category: James, M N.G.]]
-[[Category: Lu, G.J.]]
+[[Category: Lu, G J.]]
-[[Category: TBSGC, TB.Structural.Genomics.Consortium.]]
+[[Category: TBSGC, TB Structural Genomics Consortium.]]
 [[Category: amino-acid biosynthesis]]
 [[Category: arginine biosynthesis]]
@@ Line 35: / Line 35: @@
 [[Category: transcription regulation]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 23 11:00:34 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 19:07:38 2008''