2uxa: Difference between revisions
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[[Image:2uxa.gif|left|200px]]<br /><applet load="2uxa" size=" | [[Image:2uxa.gif|left|200px]]<br /><applet load="2uxa" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="2uxa, resolution 2.38Å" /> | caption="2uxa, resolution 2.38Å" /> | ||
'''CRYSTAL STRUCTURE OF THE GLUR2-FLIP LIGAND BINDING DOMAIN, R/G UNEDITED.'''<br /> | '''CRYSTAL STRUCTURE OF THE GLUR2-FLIP LIGAND BINDING DOMAIN, R/G UNEDITED.'''<br /> | ||
==Overview== | |||
The subunit composition determines AMPA receptor (AMPA-R) function and, trafficking. Mechanisms underlying channel assembly are thus central to, the efficacy and plasticity of glutamatergic synapses. We previously, showed that RNA editing at the Q/R site of the GluR2 subunit contributes, to the assembly of AMPA-R heteromers by attenuating formation of GluR2, homotetramers. Here we report that this function of the Q/R site depends, on subunit contacts between adjacent ligand binding domains (LBDs)., Changes of LBD interface contacts alter GluR2 assembly properties, forward, traffic, and expression at synapses. Interestingly, developmentally, regulated RNA editing within the LBD (at the R/G site) produces analogous, effects. Our data reveal that editing to glycine reduces the self-assembly, competence of this critical subunit and slows GluR2 maturation in the, endoplasmic reticulum (ER). Therefore, RNA editing sites, located at, strategic subunit interfaces, shape AMPA-R assembly and trafficking in a, developmentally regulated manner. | |||
==About this Structure== | ==About this Structure== | ||
2UXA is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus] with ZN and GLU as [http://en.wikipedia.org/wiki/ligands ligands]. Known structural/functional Site: <scene name='pdbsite= | 2UXA is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus] with <scene name='pdbligand=ZN:'>ZN</scene> and <scene name='pdbligand=GLU:'>GLU</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Known structural/functional Sites: <scene name='pdbsite=AC1:Zn Binding Site For Residue A 1263'>AC1</scene>, <scene name='pdbsite=AC2:Zn Binding Site For Residue B 1262'>AC2</scene>, <scene name='pdbsite=AC3:Zn Binding Site For Residue C 1263'>AC3</scene>, <scene name='pdbsite=AC4:Zn Binding Site For Residue B 1263'>AC4</scene>, <scene name='pdbsite=AC5:Zn Binding Site For Residue C 1264'>AC5</scene>, <scene name='pdbsite=AC6:GLU Binding Site For Residue A 1264'>AC6</scene>, <scene name='pdbsite=AC7:GLU Binding Site For Residue C 1265'>AC7</scene> and <scene name='pdbsite=AC8:GLU Binding Site For Residue B 1264'>AC8</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2UXA OCA]. | ||
==Reference== | |||
Developmentally regulated, combinatorial RNA processing modulates AMPA receptor biogenesis., Greger IH, Akamine P, Khatri L, Ziff EB, Neuron. 2006 Jul 6;51(1):85-97. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16815334 16815334] | |||
[[Category: Rattus norvegicus]] | [[Category: Rattus norvegicus]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: Greger, I.H.]] | [[Category: Greger, I.H.]] | ||
[[Category: Khatri, L.]] | [[Category: Khatri, L.]] | ||
[[Category: Ziff, E.]] | [[Category: Ziff, E.B.]] | ||
[[Category: GLU]] | [[Category: GLU]] | ||
[[Category: ZN]] | [[Category: ZN]] | ||
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[[Category: transport]] | [[Category: transport]] | ||
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