2c3y: Difference between revisions
No edit summary |
No edit summary |
||
| Line 1: | Line 1: | ||
[[Image:2c3y.jpg|left|200px]]<br /><applet load="2c3y" size=" | [[Image:2c3y.jpg|left|200px]]<br /><applet load="2c3y" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="2c3y, resolution 1.93Å" /> | caption="2c3y, resolution 1.93Å" /> | ||
'''CRYSTAL STRUCTURE OF THE RADICAL FORM OF PYRUVATE:FERREDOXIN OXIDOREDUCTASE FROM DESULFOVIBRIO AFRICANUS'''<br /> | '''CRYSTAL STRUCTURE OF THE RADICAL FORM OF PYRUVATE:FERREDOXIN OXIDOREDUCTASE FROM DESULFOVIBRIO AFRICANUS'''<br /> | ||
| Line 7: | Line 7: | ||
==About this Structure== | ==About this Structure== | ||
2C3Y is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Desulfovibrio_africanus Desulfovibrio africanus] with MG, CA, SF4, HTL and CO2 as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Pyruvate_synthase Pyruvate synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.2.7.1 1.2.7.1] Known structural/functional Site: <scene name='pdbsite=AC1:Co2 Binding Site For Chain B'>AC1</scene>. Full crystallographic information is available from [http:// | 2C3Y is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Desulfovibrio_africanus Desulfovibrio africanus] with <scene name='pdbligand=MG:'>MG</scene>, <scene name='pdbligand=CA:'>CA</scene>, <scene name='pdbligand=SF4:'>SF4</scene>, <scene name='pdbligand=HTL:'>HTL</scene> and <scene name='pdbligand=CO2:'>CO2</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Pyruvate_synthase Pyruvate synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.2.7.1 1.2.7.1] Known structural/functional Site: <scene name='pdbsite=AC1:Co2+Binding+Site+For+Chain+B'>AC1</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2C3Y OCA]. | ||
==Reference== | ==Reference== | ||
| Line 35: | Line 35: | ||
[[Category: tpp-dependent enzyme]] | [[Category: tpp-dependent enzyme]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Feb 3 10:31:10 2008'' | ||
Revision as of 11:31, 3 February 2008
|
CRYSTAL STRUCTURE OF THE RADICAL FORM OF PYRUVATE:FERREDOXIN OXIDOREDUCTASE FROM DESULFOVIBRIO AFRICANUS
OverviewOverview
Pyruvate-ferredoxin oxidoreductases (PFOR) are unique among thiamine, pyrophosphate (ThDP)-containing enzymes in giving rise to a rather stable, cofactor-based free-radical species upon the decarboxylation of their, first substrate, pyruvate. We have obtained snapshots of unreacted and, partially reacted (probably as a tetrahedral intermediate) pyruvate-PFOR, complexes at different time intervals. We conclude that pyruvate, decarboxylation involves very limited substrate-to-product movements but a, significant displacement of the thiazolium moiety of ThDP. In this, respect, PFOR seems to differ substantially from other ThDP-containing, enzymes, such as transketolase and pyruvate decarboxylase. In addition, exposure of PFOR to oxygen in the presence of pyruvate results in, significant inhibition of catalytic activity, both in solution and in the, crystals. Examination of the crystal structure of inhibited PFOR suggests, that the loss of activity results from oxime formation at the 4' amino, substituent of the pyrimidine moiety of ThDP.
About this StructureAbout this Structure
2C3Y is a Single protein structure of sequence from Desulfovibrio africanus with , , , and as ligands. Active as Pyruvate synthase, with EC number 1.2.7.1 Known structural/functional Site: . Full crystallographic information is available from OCA.
ReferenceReference
Flexibility of thiamine diphosphate revealed by kinetic crystallographic studies of the reaction of pyruvate-ferredoxin oxidoreductase with pyruvate., Cavazza C, Contreras-Martel C, Pieulle L, Chabriere E, Hatchikian EC, Fontecilla-Camps JC, Structure. 2006 Feb;14(2):217-24. PMID:16472741
Page seeded by OCA on Sun Feb 3 10:31:10 2008
Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)
OCA- Pages with broken file links
- Desulfovibrio africanus
- Pyruvate synthase
- Single protein
- Cavazza, C.
- Chabriere, E.
- Contreras-Martel, C.
- Fontecilla-Camps, J.C.
- Hatchikian, E.C.
- Pieulle, L.
- CA
- CO2
- HTL
- MG
- SF4
- 4fe-4s
- Electron transport
- Iron
- Iron-sulfur
- Iron-sulfur cluster
- Metal-binding
- Oxidoreductase
- Pyruvate catabolism
- Tpp-dependent enzyme