1od6: Difference between revisions
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[[Image:1od6.jpg|left|200px]]<br /><applet load="1od6" size=" | [[Image:1od6.jpg|left|200px]]<br /><applet load="1od6" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="1od6, resolution 1.5Å" /> | caption="1od6, resolution 1.5Å" /> | ||
'''THE CRYSTAL STRUCTURE OF PHOSPHOPANTETHEINE ADENYLYLTRANSFERASE FROM THERMUS THERMOPHILUS IN COMPLEX WITH 4'-PHOSPHOPANTETHEINE'''<br /> | '''THE CRYSTAL STRUCTURE OF PHOSPHOPANTETHEINE ADENYLYLTRANSFERASE FROM THERMUS THERMOPHILUS IN COMPLEX WITH 4'-PHOSPHOPANTETHEINE'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
1OD6 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus] with SO4 and PNS as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Pantetheine-phosphate_adenylyltransferase Pantetheine-phosphate adenylyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.3 2.7.7.3] Known structural/functional Site: <scene name='pdbsite=AC1:So4 Binding Site For Chain A'>AC1</scene>. Full crystallographic information is available from [http:// | 1OD6 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus] with <scene name='pdbligand=SO4:'>SO4</scene> and <scene name='pdbligand=PNS:'>PNS</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Pantetheine-phosphate_adenylyltransferase Pantetheine-phosphate adenylyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.3 2.7.7.3] Known structural/functional Site: <scene name='pdbsite=AC1:So4+Binding+Site+For+Chain+A'>AC1</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OD6 OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: transferase]] | [[Category: transferase]] | ||
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THE CRYSTAL STRUCTURE OF PHOSPHOPANTETHEINE ADENYLYLTRANSFERASE FROM THERMUS THERMOPHILUS IN COMPLEX WITH 4'-PHOSPHOPANTETHEINE
OverviewOverview
Phosphopantetheine adenylyltransferase (PPAT) is an essential enzyme in, bacteria that catalyzes the rate-limiting step in coenzyme A (CoA), biosynthesis by transferring an adenylyl group from ATP to, 4'-phosphopantetheine (Ppant), yielding 3'-dephospho-CoA (dPCoA). The, crystal structure of PPAT from Thermus thermophilus HB8 (Tt PPAT), complexed with Ppant has been determined by the molecular-replacement, method at 1.5 A resolution. The overall fold of the enzyme is almost the, same as that of Escherichia coli PPAT, a hexamer having point group 32., The asymmetric unit of Tt PPAT contains a monomer and the crystallographic, triad and dyad coincide with the threefold and twofold axes of the, hexamer, respectively. Most of the important atoms surrounding the active, site in E. coli PPAT are conserved in Tt PPAT, indicating similarities in, their substrate binding and enzymatic reaction. The notable difference, between E. coli PPAT and Tt PPAT is the simultaneous substrate recognition, by all six subunits of Tt PPAT compared with substrate recognition by only, three subunits in E. coli PPAT. Comparative analysis also revealed that, the higher stability of Tt PPAT arises from stabilization of each subunit, by hydrophobic effects, hydrogen bonds and entropic effects.
About this StructureAbout this Structure
1OD6 is a Single protein structure of sequence from Thermus thermophilus with and as ligands. Active as Pantetheine-phosphate adenylyltransferase, with EC number 2.7.7.3 Known structural/functional Site: . Full crystallographic information is available from OCA.
ReferenceReference
Structure and implications for the thermal stability of phosphopantetheine adenylyltransferase from Thermus thermophilus., Takahashi H, Inagaki E, Fujimoto Y, Kuroishi C, Nodake Y, Nakamura Y, Arisaka F, Yutani K, Kuramitsu S, Yokoyama S, Yamamoto M, Miyano M, Tahirov TH, Acta Crystallogr D Biol Crystallogr. 2004 Jan;60(Pt 1):97-104. Epub 2003, Dec 18. PMID:14684898
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Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)
OCA- Pages with broken file links
- Pantetheine-phosphate adenylyltransferase
- Single protein
- Thermus thermophilus
- Inagaki, E.
- Miyano, M.
- RSGI, RIKEN.Structural.Genomics/Proteomics.Initiative.
- Tahirov, T.
- Takahashi, H.
- PNS
- SO4
- Coenzyme a biosynthesis
- Nucleotidyltransferase
- Riken structural genomics/proteomics initiative
- Rsgi
- Structural genomics
- Transferase