1oar: Difference between revisions
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[[Image:1oar.jpg|left|200px]]<br /><applet load="1oar" size=" | [[Image:1oar.jpg|left|200px]]<br /><applet load="1oar" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="1oar, resolution 2.23Å" /> | caption="1oar, resolution 2.23Å" /> | ||
'''FV IGE SPE-7 IN COMPLEX WITH ALIZARIN RED'''<br /> | '''FV IGE SPE-7 IN COMPLEX WITH ALIZARIN RED'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
1OAR is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Rattus_rattus Rattus rattus] with CAC, NA, CL, DMS, EDO and AZN as [http://en.wikipedia.org/wiki/ligands ligands]. Known structural/functional Site: <scene name='pdbsite=AC1:Cl Binding Site For Chain N'>AC1</scene>. Full crystallographic information is available from [http:// | 1OAR is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Rattus_rattus Rattus rattus] with <scene name='pdbligand=CAC:'>CAC</scene>, <scene name='pdbligand=NA:'>NA</scene>, <scene name='pdbligand=CL:'>CL</scene>, <scene name='pdbligand=DMS:'>DMS</scene>, <scene name='pdbligand=EDO:'>EDO</scene> and <scene name='pdbligand=AZN:'>AZN</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Known structural/functional Site: <scene name='pdbsite=AC1:Cl+Binding+Site+For+Chain+N'>AC1</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OAR OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: multispecificity]] | [[Category: multispecificity]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Feb 3 09:55:40 2008'' | ||
Revision as of 10:55, 3 February 2008
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FV IGE SPE-7 IN COMPLEX WITH ALIZARIN RED
OverviewOverview
A single antibody was shown to adopt different binding-site conformations, and thereby bind unrelated antigens. Analysis by both x-ray, crystallography and pre-steady-state kinetics revealed an equilibrium, between different preexisting isomers, one of which possessed a, promiscuous, low-affinity binding site for aromatic ligands, including the, immunizing hapten. A subsequent induced-fit isomerization led to, high-affinity complexes with a deep and narrow binding site. A protein, antigen identified by repertoire selection made use of an unrelated, antibody isomer with a wide, shallow binding site. Conformational, diversity, whereby one sequence adopts multiple structures and multiple, functions, can increase the effective size of the antibody repertoire but, may also lead to autoimmunity and allergy.
About this StructureAbout this Structure
1OAR is a Single protein structure of sequence from Rattus rattus with , , , , and as ligands. Known structural/functional Site: . Full crystallographic information is available from OCA.
ReferenceReference
Antibody multispecificity mediated by conformational diversity., James LC, Roversi P, Tawfik DS, Science. 2003 Feb 28;299(5611):1362-7. PMID:12610298
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