1h8h: Difference between revisions
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[[Image:1h8h.jpg|left|200px]]<br /><applet load="1h8h" size=" | [[Image:1h8h.jpg|left|200px]]<br /><applet load="1h8h" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="1h8h, resolution 2.9Å" /> | caption="1h8h, resolution 2.9Å" /> | ||
'''BOVINE MITOCHONDRIAL F1-ATPASE CRYSTALLISED IN THE PRESENCE OF 5MM AMPPNP'''<br /> | '''BOVINE MITOCHONDRIAL F1-ATPASE CRYSTALLISED IN THE PRESENCE OF 5MM AMPPNP'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
1H8H is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus] with MG, PO4, ATP, ADP and GOL as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Transferred_entry:_3.6.3.14 Transferred entry: 3.6.3.14], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.1.34 3.6.1.34] Known structural/functional Sites: <scene name='pdbsite=AC1:Atp Binding Site For Chain A'>AC1</scene>, <scene name='pdbsite=AC2:Mg Binding Site For Chain A'>AC2</scene>, <scene name='pdbsite=AC3:Atp Binding Site For Chain B'>AC3</scene>, <scene name='pdbsite=AC4:Mg Binding Site For Chain B'>AC4</scene>, <scene name='pdbsite=AC5:Atp Binding Site For Chain C'>AC5</scene>, <scene name='pdbsite=AC6:Mg Binding Site For Chain C'>AC6</scene>, <scene name='pdbsite=AC7:Adp Binding Site For Chain D'>AC7</scene>, <scene name='pdbsite=AC8:Mg Binding Site For Chain D'>AC8</scene>, <scene name='pdbsite=AC9:Po4 Binding Site For Chain E'>AC9</scene>, <scene name='pdbsite=BC1:Atp Binding Site For Chain F'>BC1</scene>, <scene name='pdbsite=BC2:Mg Binding Site For Chain F'>BC2</scene>, <scene name='pdbsite=CAT:The Carboxylate Group Of Glutamic Aci Residue Is Believe ...'>CAT</scene> and <scene name='pdbsite=PLP:LYS Within The P-Loop (Phosphate Binding) Motif, Gxxxxgkt/S'>PLP</scene>. Full crystallographic information is available from [http:// | 1H8H is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus] with <scene name='pdbligand=MG:'>MG</scene>, <scene name='pdbligand=PO4:'>PO4</scene>, <scene name='pdbligand=ATP:'>ATP</scene>, <scene name='pdbligand=ADP:'>ADP</scene> and <scene name='pdbligand=GOL:'>GOL</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Transferred_entry:_3.6.3.14 Transferred entry: 3.6.3.14], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.1.34 3.6.1.34] Known structural/functional Sites: <scene name='pdbsite=AC1:Atp+Binding+Site+For+Chain+A'>AC1</scene>, <scene name='pdbsite=AC2:Mg+Binding+Site+For+Chain+A'>AC2</scene>, <scene name='pdbsite=AC3:Atp+Binding+Site+For+Chain+B'>AC3</scene>, <scene name='pdbsite=AC4:Mg+Binding+Site+For+Chain+B'>AC4</scene>, <scene name='pdbsite=AC5:Atp+Binding+Site+For+Chain+C'>AC5</scene>, <scene name='pdbsite=AC6:Mg+Binding+Site+For+Chain+C'>AC6</scene>, <scene name='pdbsite=AC7:Adp+Binding+Site+For+Chain+D'>AC7</scene>, <scene name='pdbsite=AC8:Mg+Binding+Site+For+Chain+D'>AC8</scene>, <scene name='pdbsite=AC9:Po4+Binding+Site+For+Chain+E'>AC9</scene>, <scene name='pdbsite=BC1:Atp+Binding+Site+For+Chain+F'>BC1</scene>, <scene name='pdbsite=BC2:Mg+Binding+Site+For+Chain+F'>BC2</scene>, <scene name='pdbsite=CAT:The+Carboxylate+Group+Of+Glutamic+Aci+Residue+Is+Believe+...'>CAT</scene> and <scene name='pdbsite=PLP:LYS+Within+The+P-Loop+(Phosphate+Binding)+Motif,+Gxxxxgkt/S'>PLP</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1H8H OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: f1fo atp synthase]] | [[Category: f1fo atp synthase]] | ||
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Revision as of 10:47, 3 February 2008
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BOVINE MITOCHONDRIAL F1-ATPASE CRYSTALLISED IN THE PRESENCE OF 5MM AMPPNP
OverviewOverview
Analysis of tryptophan mutants of F(1)-ATPase from Escherichia coli [Lobau, et al. (1997) FEBS Lett. 404, 15-18] suggested that nucleotide, concentrations used to grow crystals for the determination of the, structure of bovine F(1)-ATPase [Abrahams et al. (1994) Nature 370, 621-628] would be sufficient to occupy only two catalytic sites, and that, higher concentrations of nucleotide would result in all three sites being, occupied. We have determined the structure of bovine F(1)-ATPase at 2.9 A, resolution with crystals grown in the presence of 5 mM AMPPNP and 5 microM, ADP. Similar to previous structures of bovine F(1)-ATPase determined with, crystals grown in the presence of lower nucleotide concentrations, only, two beta-subunits have bound nucleotide and the third subunit remains, empty.
About this StructureAbout this Structure
1H8H is a Protein complex structure of sequences from Bos taurus with , , , and as ligands. Active as Transferred entry: 3.6.3.14, with EC number 3.6.1.34 Known structural/functional Sites: , , , , , , , , , , , and . Full crystallographic information is available from OCA.
ReferenceReference
The structure and nucleotide occupancy of bovine mitochondrial F(1)-ATPase are not influenced by crystallisation at high concentrations of nucleotide., Menz RI, Leslie AG, Walker JE, FEBS Lett. 2001 Apr 6;494(1-2):11-4. PMID:11297725
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