Adenylosuccinate Synthetase: Difference between revisions
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[[Image:gtp.gif|GTP]] + [[Image:imp.gif|IMP]] +[[Image:L-Aspartate.gif|L-Aspartate]] -> [[Image:GDP.gif|GDP]] + [[Image:phosphate.gif|Phosphate]] + [[Image:adenylosuccinate.gif|Adenylosuccinate]] | [[Image:gtp.gif|GTP]] + [[Image:imp.gif|IMP]] +[[Image:L-Aspartate.gif|L-Aspartate]] -> [[Image:GDP.gif|GDP]] + [[Image:phosphate.gif|Phosphate]] + [[Image:adenylosuccinate.gif|Adenylosuccinate]] | ||
The 6-O of inosine is displaced by aspartate which yields adenylosuccinate. The presence, or over abundance of AMP acts as a feedback inhibitor for AdSS.<ref>PMID:662843</ref> | The 6-O of inosine is displaced by aspartate which yields adenylosuccinate. The presence, or over abundance of AMP, GMP, GDP and adenylosuccinate acts as a feedback inhibitor for AdSS.<ref>PMID:662843</ref> | ||
Binding of GTP alone is not sufficient to induce a conformational change in the enzyme. IMP, coupled with an acetate ion invoke a conformational change which helps prepare the active site for catalysis. The reaction proceeds as follows: The gamma phosphoryl group of GTP is transferred to the 6-keto group of IMP which causes the displacement of an inorganic phosphorus molecule, Pi, from L-Aspartate. This leads to the formation of an adenylosuccinate molecule.<ref>PMID: 11560929</ref> | Binding of GTP alone is not sufficient to induce a conformational change in the enzyme. IMP, coupled with an acetate ion invoke a conformational change which helps prepare the active site for catalysis. The reaction proceeds as follows: The gamma phosphoryl group of GTP is transferred to the 6-keto group of IMP which causes the displacement of an inorganic phosphorus molecule, Pi, from L-Aspartate. This leads to the formation of an adenylosuccinate molecule.<ref>PMID: 11560929</ref> | ||