1e4v: Difference between revisions
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[[Image:1e4v.jpg|left|200px]]<br /><applet load="1e4v" size=" | [[Image:1e4v.jpg|left|200px]]<br /><applet load="1e4v" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="1e4v, resolution 1.85Å" /> | caption="1e4v, resolution 1.85Å" /> | ||
'''MUTANT G10V OF ADENYLATE KINASE FROM E. COLI, MODIFIED IN THE GLY-LOOP'''<br /> | '''MUTANT G10V OF ADENYLATE KINASE FROM E. COLI, MODIFIED IN THE GLY-LOOP'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
1E4V is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with AP5 as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Adenylate_kinase Adenylate kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.4.3 2.7.4.3] Known structural/functional Sites: <scene name='pdbsite=AC1:Ap5 Binding Site For Chain A'>AC1</scene> and <scene name='pdbsite=AC2:Ap5 Binding Site For Chain B Symmetry Related Subunits C ...'>AC2</scene>. Full crystallographic information is available from [http:// | 1E4V is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=AP5:'>AP5</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Adenylate_kinase Adenylate kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.4.3 2.7.4.3] Known structural/functional Sites: <scene name='pdbsite=AC1:Ap5 Binding Site For Chain A'>AC1</scene> and <scene name='pdbsite=AC2:Ap5 Binding Site For Chain B Symmetry Related Subunits C ...'>AC2</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1E4V OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: transferase(phosphotransferase)]] | [[Category: transferase(phosphotransferase)]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 23 09:54:33 2008'' | ||
Revision as of 10:54, 23 January 2008
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MUTANT G10V OF ADENYLATE KINASE FROM E. COLI, MODIFIED IN THE GLY-LOOP
OverviewOverview
Two mutants of adenylate kinase from Escherichia coli have been, crystallized and analyzed by X-ray diffraction at resolutions of 3.4 and, 2.4 A, respectively. These mutants are Pro-9-->Leu and Gly-10-->Val. They, were selected for their positions in the highly conserved Gly-loop forming, a giant anion hole for the beta-phosphate of ATP (GTP) in adenylate, kinases, H-ras-p21, and other nucleotide-binding proteins. Mutants at, these positions of H-ras-p21 cause cancer. In adenylate kinase these, mutations cause smallish changes at the active site. Relating the, structural changes to the known changes in catalysis indicates that these, mutants hinder the induced-fit movements. As a side result we find that, mutant Pro-9-->Leu and wild-type form one very similar crystal packing, contact that is crystallographic in one case and noncrystallographic in, the other, while all other packing contacts and the space groups are quite, at variance.
About this StructureAbout this Structure
1E4V is a Single protein structure of sequence from Escherichia coli with as ligand. Active as Adenylate kinase, with EC number 2.7.4.3 Known structural/functional Sites: and . Full crystallographic information is available from OCA.
ReferenceReference
Crystal structures of two mutants of adenylate kinase from Escherichia coli that modify the Gly-loop., Muller CW, Schulz GE, Proteins. 1993 Jan;15(1):42-9. PMID:8451239
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