1bui: Difference between revisions
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[[Image:1bui.jpg|left|200px]]<br /><applet load="1bui" size=" | [[Image:1bui.jpg|left|200px]]<br /><applet load="1bui" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="1bui, resolution 2.65Å" /> | caption="1bui, resolution 2.65Å" /> | ||
'''STRUCTURE OF THE TERNARY MICROPLASMIN-STAPHYLOKINASE-MICROPLASMIN COMPLEX: A PROTEINASE-COFACTOR-SUBSTRATE COMPLEX IN ACTION.'''<br /> | '''STRUCTURE OF THE TERNARY MICROPLASMIN-STAPHYLOKINASE-MICROPLASMIN COMPLEX: A PROTEINASE-COFACTOR-SUBSTRATE COMPLEX IN ACTION.'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
1BUI is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Active as [http://en.wikipedia.org/wiki/Plasmin Plasmin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.7 3.4.21.7] Known structural/functional Sites: <scene name='pdbsite=ASA:Catalytic Site Of Chain A'>ASA</scene> and <scene name='pdbsite=ASB:Catalytic Site Of Chain B'>ASB</scene>. Full crystallographic information is available from [http:// | 1BUI is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Active as [http://en.wikipedia.org/wiki/Plasmin Plasmin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.7 3.4.21.7] Known structural/functional Sites: <scene name='pdbsite=ASA:Catalytic+Site+Of+Chain+A'>ASA</scene> and <scene name='pdbsite=ASB:Catalytic+Site+Of+Chain+B'>ASB</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BUI OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: staphylokinase]] | [[Category: staphylokinase]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Feb 3 09:34:02 2008'' | ||
Revision as of 10:34, 3 February 2008
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STRUCTURE OF THE TERNARY MICROPLASMIN-STAPHYLOKINASE-MICROPLASMIN COMPLEX: A PROTEINASE-COFACTOR-SUBSTRATE COMPLEX IN ACTION.
OverviewOverview
The serine proteinase plasmin is the key fibrinolytic enzyme that, dissolves blood clots and also promotes cell migration and tissue, remodeling. Here, we report the 2.65 A crystal structure of a ternary, complex of microplasmin-staphylokinase bound to a second microplasmin. The, staphylokinase 'cofactor' does not affect the active-site geometry of the, plasmin 'enzyme', but instead modifies its subsite specificity by, providing additional docking sites for enhanced presentation of the, plasminogen 'substrate' to the 'enzymes's' active site. The activation, loop of the plasmin 'substrate', cleaved in these crystals, can be, reconstructed to show how it runs across the active site of the plasmin, 'enzyme' prior to activation cleavage. This is the first experimental, structure of a productive proteinase-cofactor-macromolecular substrate, complex. Furthermore, it provides a template for the design of improved, plasminogen activators and plasmin inhibitors with considerable, therapeutical potential.
DiseaseDisease
Known diseases associated with this structure: Conjunctivitis, ligneous OMIM:[173350], Plasminogen Tochigi disease OMIM:[173350], Plasminogen deficiency, types I and II OMIM:[173350], Thrombophilia, dysplasminogenemic OMIM:[173350]
About this StructureAbout this Structure
1BUI is a Protein complex structure of sequences from Homo sapiens. Active as Plasmin, with EC number 3.4.21.7 Known structural/functional Sites: and . Full crystallographic information is available from OCA.
ReferenceReference
The ternary microplasmin-staphylokinase-microplasmin complex is a proteinase-cofactor-substrate complex in action., Parry MA, Fernandez-Catalan C, Bergner A, Huber R, Hopfner KP, Schlott B, Guhrs KH, Bode W, Nat Struct Biol. 1998 Oct;5(10):917-23. PMID:9783753
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