Fructose Bisphosphate Aldolase: Difference between revisions
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Fructose biphosphate aldolase is an enzyme in glycolysis. It catalyzes the breakdown of fructose-1,6-biophosphate into dihydroxyacetone phosphate (DHAP) and glyceraldehyde-2-phosphate (GAP). The reaction is an aldol cleavage, or otherwise termed, retro aldo condensation. Catalysis occurs by the formation of a Schiff base (an imine resulting from a ketone and amine) from the amine of the aldolase's Lys229 and the open-ring form of FBP accompanied by stabilization from Asp33. <scene name='Austin_Drake_Sandbox/Catalytic_site/1'>Catalytic Site</scene> aldol cleavage produces GAP and an enamine precursor to DHAP. Tautomerization, protonation and the hydrolysis of the Schiff base produce the final product of DHAP and the active enzyme. | Fructose biphosphate aldolase is an enzyme in glycolysis. It catalyzes the breakdown of fructose-1,6-biophosphate into dihydroxyacetone phosphate (DHAP) and glyceraldehyde-2-phosphate (GAP). The reaction is an aldol cleavage, or otherwise termed, retro aldo condensation. Catalysis occurs by the formation of a Schiff base (an imine resulting from a ketone and amine) from the amine of the aldolase's Lys229 and the open-ring form of FBP accompanied by stabilization from Asp33. <scene name='Austin_Drake_Sandbox/Catalytic_site/1'>Catalytic Site</scene> aldol cleavage produces GAP and an enamine precursor to DHAP. Tautomerization, protonation and the hydrolysis of the Schiff base produce the final product of DHAP and the active enzyme. | ||
<scene name='Austin_Drake_Sandbox/B_sheet_barrel/2'> | <scene name='Austin_Drake_Sandbox/B_sheet_barrel/2'>FBP fits here</scene> | ||
{{STRUCTURE_2ald | PDB=2ald | SCENE= }} | {{STRUCTURE_2ald | PDB=2ald | SCENE= }} | ||
Jmol: an open-source Java viewer for chemical structures in 3D. http://www.jmol.org/ | Jmol: an open-source Java viewer for chemical structures in 3D. http://www.jmol.org/ | ||